Dou Lei, Zhang Yi
Department of Gynecology, The First Affiliated Hospital of China Medical University, Shenyang, China.
Front Oncol. 2021 Mar 9;11:614035. doi: 10.3389/fonc.2021.614035. eCollection 2021.
microRNAs (miRNAs) are of great significance in cancer treatment, which may have a desirable result on the regulation of tumorigenesis, progression, recurrence, and chemo-resistance of ovarian cancer. However, the research on the further potential application of miR-4461 in ovarian cancer is little and limited. Therefore, the study in this paper focus on the investigation of the of miR-4461 in ovarian cancer progression and chemo-resistance. The phenomenon that the proliferation and metastasis of ovarian cancer cells can be promoted by miR4461 is revealed in functional assays. Through the bioinformatics and luciferase reporter analysis, the PTEN is validated to be the direct target of miR-4461 in ovarian. The association between the expression of miR-4461 and PTEN is negative in in human ovarian cancer tissues. The distinction of growth and metastasis capacity between miR-4461 knockdown ovarian cancer cells and control cells is partially abolished by si-PTEN. Moreover, it was found that cisplatin treatment has obvious effect on the miR-4461 knockdown ovarian cancer cells. In summary, the data given in this paper indicate that the miR-4461 can be regarded as a potential onco-miRNA in ovarian cancer by targeting PTEN.
微小RNA（miRNA）在癌症治疗中具有重要意义，其可能对卵巢癌的肿瘤发生、进展、复发和化疗耐药性的调节产生理想效果。然而，关于miR-4461在卵巢癌中进一步潜在应用的研究很少且有限。因此，本文的研究重点是调查miR-4461在卵巢癌进展和化疗耐药性中的作用。功能试验揭示了miR-4461可促进卵巢癌细胞增殖和转移的现象。通过生物信息学和荧光素酶报告基因分析，验证了PTEN是卵巢中miR-4461的直接靶点。在人卵巢癌组织中，miR-4461与PTEN的表达呈负相关。si-PTEN部分消除了miR-4461敲低的卵巢癌细胞与对照细胞之间生长和转移能力的差异。此外，发现顺铂处理对miR-4461敲低的卵巢癌细胞有明显作用。总之，本文给出的数据表明，miR-4461通过靶向PTEN可被视为卵巢癌中一种潜在的致癌miRNA。
