Kyu L N, Kanamaru H, Yoshida O
Department of Urology, Faculty of Medicine, Kyoto University.
Hinyokika Kiyo. 1988 Jan;34(1):32-6.
The effect of radiation on the human bladder cancer cell lines, KK47 and J82, was studied using colony formation assay and [3H] thymidine incorporation assay. The response to radiation was dose-related for both assays, but the curve for the colony formation assay was steeper than that for [3H] thymidine incorporation assay. However, the steepness of the curve for [3H] thymidine incorporation assay increased to be approximately the same as that for the colony formation assay as the incubation period increased. [3H] Thymidine incorporation assay can provide a rapid assessment of radiation sensitivity comparable to that obtained by the colony formation assay to select a radiosensitive bladder cancer patient for definitive or adjunctive radiotherapy.
利用集落形成试验和[3H]胸苷掺入试验研究了辐射对人膀胱癌细胞系KK47和J82的影响。两种试验中，辐射反应均与剂量相关，但集落形成试验的曲线比[3H]胸苷掺入试验的曲线更陡。然而，随着孵育时间的增加，[3H]胸苷掺入试验曲线的陡度增加，与集落形成试验的曲线大致相同。[3H]胸苷掺入试验能够快速评估辐射敏感性，与通过集落形成试验获得的结果相当，从而为选择适合根治性或辅助性放疗的放射敏感性膀胱癌患者提供依据。
