Kurth K H, Romijn J C, Kappendijk M, Weissglas M G, van Dongen J W
Department of Urology, Erasmus University Rotterdam, The Netherlands.
Semin Surg Oncol. 1988;4(2):103-9.
In vivo and in vitro chemosensitivity testing has been applied to kidney carcinoma tumor lines. Serially transplantable tumors were implanted subcutaneously in nude mice and the animals were treated with cytostatic drugs. The results of this in vivo assay were compared with the results obtained with the subrenal capsule assay, the DNA precursor assay (3H-thymidine), and the colony-formation assay, utilizing the same tumor line in each case. Higher rates of resistant tumors were found in the in vivo assays than in the in vitro assays. The SRC assay and the DNA assay had the highest predictive value, as judged from comparison with the results obtained with the source tumor (human kidney carcinoma tumor line).
体内和体外化学敏感性测试已应用于肾癌肿瘤细胞系。将可连续移植的肿瘤皮下植入裸鼠体内，并用细胞抑制药物对动物进行治疗。将这种体内试验的结果与肾被膜下试验、DNA前体试验（3H-胸腺嘧啶核苷）以及集落形成试验的结果进行比较，每种情况下均使用相同的肿瘤细胞系。体内试验中发现的耐药肿瘤发生率高于体外试验。从与源肿瘤（人肾癌肿瘤细胞系）获得的结果比较来看，肾被膜下试验和DNA试验具有最高的预测价值。
