Makvandi Pooyan, Ashrafizadeh Milad, Ghomi Matineh, Najafi Masoud, Hossein Hamid Heydari Sheikh, Zarrabi Ali, Mattoli Virgilio, Varma Rajender S
Istituto Italiano di Tecnologia, Centre for Materials Interface, viale Rinaldo Piaggio 34, 56025, Pontedera, Pisa, Italy.
Department of Basic Science, Faculty of Veterinary Medicine, University of Tabriz, 51666-16471, Tabriz, Iran.
Prog Biomater. 2021 Mar;10(1):77-89. doi: 10.1007/s40204-021-00155-6. Epub 2021 Mar 26.
Injectable materials have shown great potential in tissue engineering applications. However, bacterial infection is one of the main challenges in using these materials in the field of regenerative medicine. In this study, biogenically synthesized silver nanoparticle-decorated multi-walled carbon nanotubes (Ag/MWCNTs) were deployed for adorning biogenic-derived AgNPs which were subsequently used in the preparation of thermosensitive hydrogels based on hyaluronic acid encompassing these green-synthesized NPs. The antibacterial capacity of AgNPs decorated on MWCNTs synthesized through Camellia sinensis extract in an organic solvent-free medium displayed a superior activity by inhibiting the growth of Gram-negative (E. coli and Klebsiella) and Gram-positive (S. aureus and E. faecalis). The injectable hydrogel nanocomposites demonstrated good mechanical properties, as well. The thermosensitive hyaluronic acid-based hydrogels also exhibited T below the body temperature, indicating the transition from liquid-like behavior to elastic gel-like behavior. Such a promising injectable nanocomposite could be applied as liquid, pomade, or ointment to enter wound cavities or bone defects and subsequently its transition in situ to gel form at human body temperature bodes well for their immense potential application in the biomedical sector.
可注射材料在组织工程应用中显示出巨大潜力。然而，细菌感染是在再生医学领域使用这些材料的主要挑战之一。在本研究中，采用生物合成的银纳米颗粒修饰的多壁碳纳米管（Ag/MWCNTs）来装饰生物源衍生的AgNPs，随后将其用于制备基于透明质酸的热敏水凝胶，其中包含这些绿色合成的纳米颗粒。在无有机溶剂的介质中通过茶树提取物合成的MWCNTs上装饰的AgNPs的抗菌能力通过抑制革兰氏阴性菌（大肠杆菌和克雷伯氏菌）和革兰氏阳性菌（金黄色葡萄球菌和粪肠球菌）的生长表现出优异的活性。可注射水凝胶纳米复合材料也表现出良好的机械性能。基于透明质酸的热敏水凝胶在体温以下也表现出相转变温度，表明从类似液体的行为转变为弹性凝胶状行为。这种有前景的可注射纳米复合材料可以以液体、软膏或药膏的形式应用于进入伤口腔或骨缺损部位，随后在人体温度下原位转变为凝胶形式，预示着它们在生物医学领域具有巨大的潜在应用价值。
