School of Life Sciences, Northwestern Polytechnical University, Xi'an, P. R. China.
Key Laboratory for Space Bioscience and Biotechnology, Institute of Special Environment Biophysics, Northwestern Polytechnical University, Xi'an, P. R. China.
Electrophoresis. 2021 Jul;42(12-13):1340-1351. doi: 10.1002/elps.202000291. Epub 2021 Apr 1.
One of the most cited limitations of biochemical detection is its poor sensitivity, owing to the relatively high complexity of micro-samples. Moreover, some samples cannot be easily self-replicated and their abundance cannot be increased through traditional technologies. Therefore, the preconcentration of low-abundance samples is a key requirement for microfluidic biological analysis. In recent years, the ion-concentration polarization phenomenon has aroused widespread interest in the application of microfluidic technology. In addition, paper-based materials are readily available, easy to modify, and exhibit good hydrophilicity. The study of the ion-concentration polarization preconcentration of micro-samples in paper-based microfluidic chips is of considerable significance. In this review, we discuss the development and applications of ion-concentration polarization paper-based preconcentrator in the past 5 years, with emphasis on key progresses in chip fabrication and performance optimization under different conditions. The current needs and development prospects in this field have also been discussed.
生化检测的最主要局限性之一是其灵敏度较差，这是由于微样本的相对复杂性所致。此外，一些样本不容易自我复制，其丰度也不能通过传统技术增加。因此，对低丰度样本的预浓缩是微流控生物分析的关键要求。近年来，离子浓度极化现象在微流控技术的应用中引起了广泛关注。此外，纸基材料易于获得、易于修饰且具有良好的亲水性。研究微流控芯片中基于纸的微样本的离子浓度极化预浓缩具有重要意义。在这篇综述中，我们讨论了过去 5 年中离子浓度极化基于纸的预浓缩器在微流控技术中的发展和应用，重点介绍了在不同条件下在芯片制造和性能优化方面的关键进展。还讨论了该领域的当前需求和发展前景。
