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评估与 miR-144 和 miR-34a 过表达相关的 medullary 甲状腺癌 (MTC) 细胞系中 和 基因的表达。

Assessment of and genes expression in medullary thyroid carcinoma (MTC) cell line in relation with over expression of miR-144 and miR-34a.

机构信息

Department of Medicine, Tehran Medical Sciences Branch, Islamic Azad University, Tehran, Iran.

Department of Biochemistry, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.

出版信息

Horm Mol Biol Clin Investig. 2021 Mar 26;42(3):265-271. doi: 10.1515/hmbci-2020-0050.

DOI:10.1515/hmbci-2020-0050
PMID:33769725
Abstract

OBJECTIVES

The aim of the present study was to investigate the expression of and genes and their targeting microRNAs (miRNAs) including miR-34a and miR-144 in Medullary Thyroid Carcinoma (MTC) cell line, TT, and determine the effect of these two miRNAs on their target genes to introduce new molecular markers or therapeutics.

METHODS

The expression of miR-34a, miR-144, and their targets genes including and was evaluated by quantitative Real-time PCR. Luciferase assay was performed to confirm the interaction between miRNAs and their target mRNAs. The expression level of and was evaluated before and after miRNAs induction in TT cell line compared with Cos7 as control cells.

RESULTS

The expression of and were up-regulated significantly, while miR-34a and miR-144 were down-regulated in TT cell line compared to Cos7. After transduction, the overexpression of miR-34a and 144 caused down-regulation of both genes. Luciferase assay results showed that the is targeted by miR-34a and miR-144 and the intensity of luciferase decreased in the presence of miRNAs.

CONCLUSIONS

Based on the results of the present study and since and genes play a critical role in variety of human cancers, suppression of these genes by their targeting miRNAs, especially miR-34a and miR-144, can be propose as a new strategy for MTC management. However, more studies are needed to approve the hypothesis.

摘要

目的

本研究旨在探讨 和 基因及其靶向 microRNAs（miRNAs），包括 miR-34a 和 miR-144 在甲状腺髓样癌（MTC）细胞系 TT 中的表达，并确定这两种 miRNA 对其靶基因的影响，以引入新的分子标志物或治疗方法。

方法

通过定量实时 PCR 评估 miR-34a、miR-144 及其靶基因 和 的表达。通过荧光素酶报告基因实验验证 miRNA 与其靶 mRNA 之间的相互作用。在 TT 细胞系中，与 Cos7 作为对照细胞相比，评估 miRNA 诱导前后 和 基因的表达水平。

结果

与 Cos7 相比，TT 细胞系中 和 的表达显著上调，而 miR-34a 和 miR-144 的表达下调。转导后，miR-34a 和 144 的过表达导致这两个基因的下调。荧光素酶报告基因实验结果表明， 是 miR-34a 和 miR-144 的靶基因，在存在 miRNA 时，荧光素酶的强度降低。

结论

基于本研究的结果，由于 和 基因在多种人类癌症中发挥关键作用，通过其靶向 miRNAs（尤其是 miR-34a 和 miR-144）抑制这些基因可以作为 MTC 管理的新策略。然而，需要更多的研究来验证这一假设。