Assessment of and genes expression in medullary thyroid carcinoma (MTC) cell line in relation with over expression of miR-144 and miR-34a.

OBJECTIVES

The aim of the present study was to investigate the expression of and genes and their targeting microRNAs (miRNAs) including miR-34a and miR-144 in Medullary Thyroid Carcinoma (MTC) cell line, TT, and determine the effect of these two miRNAs on their target genes to introduce new molecular markers or therapeutics.

METHODS

The expression of miR-34a, miR-144, and their targets genes including and was evaluated by quantitative Real-time PCR. Luciferase assay was performed to confirm the interaction between miRNAs and their target mRNAs. The expression level of and was evaluated before and after miRNAs induction in TT cell line compared with Cos7 as control cells.

RESULTS

The expression of and were up-regulated significantly, while miR-34a and miR-144 were down-regulated in TT cell line compared to Cos7. After transduction, the overexpression of miR-34a and 144 caused down-regulation of both genes. Luciferase assay results showed that the is targeted by miR-34a and miR-144 and the intensity of luciferase decreased in the presence of miRNAs.

CONCLUSIONS

Based on the results of the present study and since and genes play a critical role in variety of human cancers, suppression of these genes by their targeting miRNAs, especially miR-34a and miR-144, can be propose as a new strategy for MTC management. However, more studies are needed to approve the hypothesis.