Laboratory of Bacteriology and Virology, Hospital Aristide Le Dantec, Dakar, Senegal.
Institute for Health Research, Epidemiological Surveillance and Training (IRESSEF), Dakar, Senegal.
PLoS One. 2021 Mar 26;16(3):e0249357. doi: 10.1371/journal.pone.0249357. eCollection 2021.
Delayed Plasmodium falciparum parasite clearance has been associated with Single Nucleotide Polymorphisms (SNPs) in the kelch protein propeller domain (coded by pfk13 gene). SNPs in the Plasmodium falciparum multidrug resistance gene 1 (pfmdr1) are associated with multi-drug resistance including the combination artemether-lumefantrine. To our knowledge, this is the first work providing information on the prevalence of k13-propeller and pfmdr1 mutations from Sédhiou, a region in the south of Senegal.
147 dried blood spots on filter papers were collected from symptomatic patients attending a hospital located in Bounkiling City, Sédhiou Region, Southern Senegal. All samples were collected between 2015-2017 during the malaria transmission season. Specific regions of the gene pfk13 and pfmdr1 were analyzed using PCR amplification and Sanger sequencing.
The majority of parasites (92.9%) harboured the pfk13 wild type sequence and 6 samples harboured synonymous changes. Regarding pfmdr1, wild-type alleles represented the majority except at codon 184. Overall, prevalence of 86Y was 11.9%, 184F was 56.3% and 1246Y was 1.5%. The mutant allele 184F decreased from 73.7% in 2015 to 40.7% in 2017. The prevalence of haplotype NFD decreased from 71.4% in 2015 to 20.8% in 2017.
This study provides the first description of pfk13 and pfmdr1 genes variations in Bounkiling, a city in the Sédhiou Region of Senegal, contributing to closing the gap of information on anti-malaria drug resistance molecular markers in southern Senegal.
疟原虫清除延迟与kelch 蛋白螺旋桨结构域中的单核苷酸多态性（SNP）有关（由 pfk13 基因编码）。疟原虫多药耐药基因 1（pfmdr1）中的 SNP 与多药耐药有关，包括青蒿琥酯-咯萘啶联合用药。据我们所知，这是首次从塞内加尔南部的塞迪奥地区的布恩基林市医院就诊的有症状患者中提供有关 k13-螺旋桨和 pfmdr1 突变流行率的信息。
2015-2017 年疟疾传播季节，在塞内加尔南部塞迪奥地区布恩基林市的一家医院，采集了 147 个滤纸干血斑。所有样本均来自就诊的有症状患者。使用 PCR 扩增和 Sanger 测序分析 pfk13 和 pfmdr1 基因的特定区域。
大多数寄生虫（92.9%）携带 pfk13 野生型序列，有 6 个样本携带同义突变。关于 pfmdr1，野生型等位基因占多数，除了 184 密码子。总的来说，86Y 的流行率为 11.9%，184F 为 56.3%，1246Y 为 1.5%。突变等位基因 184F 从 2015 年的 73.7%下降到 2017 年的 40.7%。NFD 单倍型的流行率从 2015 年的 71.4%下降到 2017 年的 20.8%。
本研究首次描述了塞内加尔塞迪奥地区布恩基林市 pfk13 和 pfmdr1 基因的变异情况，有助于填补塞内加尔南部抗疟药物耐药分子标记信息的空白。
