Moriano Alessandro, Serra Diego O, Hoard Amparo, Montaña Sabrina, Degrossi Jose, Bonomo Robert A, Papp-Wallace Krisztina M, Ramirez Maria Soledad
Department of Biological Science, California State University, 800 N State College Blvd, Fullerton, CA, 92831, USA.
Instituto de Biología Molecular y Celular de Rosario (IBR, CONICET-UNR), Rosario, Argentina.
Curr Microbiol. 2021 May;78(5):1864-1870. doi: 10.1007/s00284-021-02458-0. Epub 2021 Mar 26.
Polymicrobial lung infections in individuals with Cystic Fibrosis (CF) contribute to the complexity of this disease and are a major cause of morbidity and mortality in the CF community. The microorganisms most commonly associated with severe airway infections in individuals with CF are the opportunistic pathogens S. aureus, P. aeruginosa and bacteria from the Burkholderia cepacia complex (Bcc), particularly B. cenocepacia and B. multivorans. Three Bcc strains, two S. aureus wild-type strains, and two derivative mutants were used to investigate the interplay between S. aureus and Bcc with a focus on the hemolytic activity of Bcc. Our results revealed that extracellular products from S. aureus potentiated the hemolysis of Bcc strains. Moreover, this effect was influenced by the composition of the medium in which S. aureus is grown. These findings contribute towards the understanding of the impact of interactions between S. aureus and Bcc and their possible implications in the context of co-infections by these pathogens in individuals with CF.
囊性纤维化（CF）患者的肺部多重微生物感染增加了该疾病的复杂性，是CF患者群体发病和死亡的主要原因。与CF患者严重气道感染最常相关的微生物是机会性病原体金黄色葡萄球菌、铜绿假单胞菌以及洋葱伯克霍尔德菌复合体（Bcc）中的细菌，特别是洋葱伯克霍尔德菌和多噬伯克霍尔德菌。使用三株Bcc菌株、两株金黄色葡萄球菌野生型菌株和两株衍生突变体来研究金黄色葡萄球菌和Bcc之间的相互作用，重点是Bcc的溶血活性。我们的结果表明，金黄色葡萄球菌的细胞外产物增强了Bcc菌株的溶血作用。此外，这种效应受金黄色葡萄球菌生长培养基成分的影响。这些发现有助于理解金黄色葡萄球菌和Bcc之间相互作用的影响及其在CF患者中这些病原体合并感染情况下的可能意义。
