• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

用于检测甲型禽流感病毒（H7N9）血凝素及预防病毒感染的小鼠单克隆抗体的研制。

Development of mouse monoclonal antibody for detecting hemagglutinin of avian influenza A(H7N9) virus and preventing virus infection.

作者信息

Chiang Yi-Wei, Li Chia-Jung, Su Heng-Yi, Hsieh Kai-Ting, Weng Chia-Wei, Chen Hui-Wen, Chang Shih-Chung

机构信息

Department of Biochemical Science and Technology, College of Life Science, National Taiwan University, Taipei, 106, Taiwan.

Department of Veterinary Medicine, National Taiwan University, Taipei, 106, Taiwan.

出版信息

Appl Microbiol Biotechnol. 2021 Apr;105(8):3235-3248. doi: 10.1007/s00253-021-11253-7. Epub 2021 Mar 26.

DOI:10.1007/s00253-021-11253-7
PMID:33770244
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7995400/
Abstract

Many cases of avian influenza A(H7N9) virus infection in humans have been reported since its first emergence in 2013. The disease is of concern because most patients have become severely ill with roughly 30% mortality rate. Because the threat in public health caused by H7N9 virus remains high, advance preparedness is essentially needed. In this study, the recombinant H7N9 hemagglutinin (HA) was expressed in insect cells and purified for generation of two monoclonal antibodies, named F3-2 and 1C6B. F3-2 can only recognize the H7N9 HA without having cross-reactivity with HA proteins of H1N1, H3N2, H5N1, and H7N7. 1C6B has the similar specificity with F3-2, but 1C6B can also bind to H7N7 HA. The binding epitope of F3-2 is mainly located in the region of H7N9 HA(299-307). The binding epitope of 1C6B is located in the region of H7N9 HA(489-506). F3-2 and 1C6B could not effectively inhibit the hemagglutination activity of H7N9 HA. However, F3-2 can prevent H7N9 HA from trypsin cleavage and can bind to H7N9 HA which has undergone pH-induced conformational change. F3-2 also has the ability of binding to H7N9 viral particles and inhibiting H7N9 virus infection to MDCK cells with the IC50 value of 22.18 μg/mL. In addition, F3-2 and 1C6B were utilized for comprising a lateral flow immunochromatographic test strip for specific detection of H7N9 HA. KEY POINTS: • Two mouse monoclonal antibodies, F3-2 and 1C6B, were generated for recognizing the novel binding epitopes in H7N9 HA. • F3-2 can prevent H7N9 HA from trypsin cleavage and inhibit H7N9 virus infection to MDCK cells. • F3-2 and 1C6B were developed as a lateral flow immunochromatographic test for specific detection of H7N9 HA.

摘要

自2013年首次出现以来，已报告了多例人感染甲型H7N9禽流感病毒的病例。该疾病令人担忧，因为大多数患者病情严重，死亡率约为30%。由于H7N9病毒对公共卫生造成的威胁仍然很高，因此迫切需要提前做好准备。在本研究中，重组H7N9血凝素（HA）在昆虫细胞中表达并纯化，用于制备两种单克隆抗体，分别命名为F3-2和1C6B。F3-2只能识别H7N9 HA，与H1N1、H3N2、H5N1和H7N7的HA蛋白无交叉反应。1C6B与F3-2具有相似的特异性，但1C6B也能与H7N7 HA结合。F3-2的结合表位主要位于H7N9 HA（299-307）区域。1C6B的结合表位位于H7N9 HA（489-506）区域。F3-2和1C6B不能有效抑制H7N9 HA的血凝活性。然而，F3-2可以阻止H7N9 HA被胰蛋白酶切割，并能与经历pH诱导构象变化的H7N9 HA结合。F3-2还具有结合H7N9病毒颗粒和抑制H7N9病毒感染MDCK细胞的能力，IC50值为22.18μg/mL。此外，F3-2和1C6B被用于构建一种侧向流动免疫层析试纸条，用于特异性检测H7N9 HA。

关键点

• 制备了两种小鼠单克隆抗体F3-2和1C6B，用于识别H7N9 HA中的新型结合表位。

• F3-2可以阻止H7N9 HA被胰蛋白酶切割，并抑制H7N9病毒感染MDCK细胞。

• F3-2和1C6B被开发为一种侧向流动免疫层析试验，用于特异性检测H7N9 HA。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/72d3/7995400/8f36cbefaf96/253_2021_11253_Fig10_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/72d3/7995400/2544e8f5a6ca/253_2021_11253_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/72d3/7995400/57b822039948/253_2021_11253_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/72d3/7995400/9541c678eb01/253_2021_11253_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/72d3/7995400/8ce06cbe07e0/253_2021_11253_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/72d3/7995400/7f4b2b6314c4/253_2021_11253_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/72d3/7995400/5a97ab67fde9/253_2021_11253_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/72d3/7995400/3d1668f79287/253_2021_11253_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/72d3/7995400/f566d63504f3/253_2021_11253_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/72d3/7995400/0421e713afe6/253_2021_11253_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/72d3/7995400/8f36cbefaf96/253_2021_11253_Fig10_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/72d3/7995400/2544e8f5a6ca/253_2021_11253_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/72d3/7995400/57b822039948/253_2021_11253_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/72d3/7995400/9541c678eb01/253_2021_11253_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/72d3/7995400/8ce06cbe07e0/253_2021_11253_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/72d3/7995400/7f4b2b6314c4/253_2021_11253_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/72d3/7995400/5a97ab67fde9/253_2021_11253_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/72d3/7995400/3d1668f79287/253_2021_11253_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/72d3/7995400/f566d63504f3/253_2021_11253_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/72d3/7995400/0421e713afe6/253_2021_11253_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/72d3/7995400/8f36cbefaf96/253_2021_11253_Fig10_HTML.jpg

相似文献

1
Development of mouse monoclonal antibody for detecting hemagglutinin of avian influenza A(H7N9) virus and preventing virus infection.用于检测甲型禽流感病毒（H7N9）血凝素及预防病毒感染的小鼠单克隆抗体的研制。
Appl Microbiol Biotechnol. 2021 Apr;105(8):3235-3248. doi: 10.1007/s00253-021-11253-7. Epub 2021 Mar 26.
2
Development and biochemical characterization of the monoclonal antibodies for specific detection of the emerging H5N8 and H5Nx avian influenza virus hemagglutinins.用于特异性检测新出现的H5N8和H5Nx禽流感病毒血凝素的单克隆抗体的开发及生化特性分析
Appl Microbiol Biotechnol. 2021 Jan;105(1):235-245. doi: 10.1007/s00253-020-11035-7. Epub 2020 Nov 27.
3
Development of rapid immunochromatographic test for hemagglutinin antigen of H7 subtype in patients infected with novel avian influenza A (H7N9) virus.新型 H7N9 禽流感病毒感染患者血凝素抗原的快速免疫层析检测方法的建立。
PLoS One. 2014 Mar 19;9(3):e92306. doi: 10.1371/journal.pone.0092306. eCollection 2014.
4
Development and optimized pairing of mouse monoclonal antibodies for detecting hemagglutinin in novel H7 subtype influenza viruses.开发并优化配对用于检测新型 H7 亚型流感病毒血凝素的鼠源单克隆抗体。
Sci China Life Sci. 2020 Feb;63(2):279-289. doi: 10.1007/s11427-018-9486-0. Epub 2019 May 28.
5
A broadly neutralizing human monoclonal antibody against the hemagglutinin of avian influenza virus H7N9.一种针对禽流感病毒 H7N9 血凝素的广谱中和人源单克隆抗体。
Chin Med J (Engl). 2022 Apr 5;135(7):799-805. doi: 10.1097/CM9.0000000000002001.
6
[Characterization of a monoclonal antibody against the hemagglutinin stem of H7N9 subtype avian influenza virus].[抗H7N9亚型禽流感病毒血凝素茎区单克隆抗体的鉴定]
Sheng Wu Gong Cheng Xue Bao. 2022 Jan 25;38(1):160-173. doi: 10.13345/j.cjb.210173.
7
Generation and Characterization of Monoclonal Antibodies Specific to Avian Influenza H5N1 Hemagglutinin Protein.针对禽流感H5N1血凝素蛋白的单克隆抗体的产生与特性分析
Monoclon Antib Immunodiagn Immunother. 2015 Dec;34(6):436-41. doi: 10.1089/mab.2015.0047.
8
Specific Monoclonal Antibodies Targeting Unique HA Epitopes Block H7N9 Influenza A Viral Replication.特异性单克隆抗体针对独特的 HA 表位阻断 H7N9 流感病毒复制。
J Virol. 2022 Sep 28;96(18):e0123822. doi: 10.1128/jvi.01238-22. Epub 2022 Aug 29.
9
Inactivated H7 Influenza Virus Vaccines Protect Mice despite Inducing Only Low Levels of Neutralizing Antibodies.灭活H7流感病毒疫苗尽管仅诱导产生低水平的中和抗体，但仍可保护小鼠。
J Virol. 2017 Sep 27;91(20). doi: 10.1128/JVI.01202-17. Print 2017 Oct 15.
10
Identification of H7N9 hemagglutinin novel protein epitopes that elicit strong antibody-dependent, cell-mediated cytotoxic activities with protection from influenza infection in mouse model.鉴定 H7N9 血凝素新型蛋白表位，这些表位能够引发强烈的抗体依赖的、细胞介导的细胞毒性活性，并能在小鼠模型中预防流感感染。
Cell Immunol. 2021 Jan;359:104255. doi: 10.1016/j.cellimm.2020.104255. Epub 2020 Dec 6.

引用本文的文献

1
Cross-Reactive Fc-Fused Single-Domain Antibodies to Hemagglutinin Stem Region Protect Mice from Group 1 Influenza a Virus Infection.针对血凝素茎部区域的交叉反应性 Fc 融合单域抗体可保护小鼠免受 1 型流感病毒感染。
Viruses. 2022 Nov 10;14(11):2485. doi: 10.3390/v14112485.
2
Neutralization or enhancement of SARS-CoV-2 infection by a monoclonal antibody targeting a specific epitope in the spike receptor-binding domain.针对刺突受体结合域中特定表位的单克隆抗体对 SARS-CoV-2 感染的中和或增强作用。
Antiviral Res. 2022 Apr;200:105290. doi: 10.1016/j.antiviral.2022.105290. Epub 2022 Mar 13.
3
Neutralizing Monoclonal Antibodies Inhibit SARS-CoV-2 Infection through Blocking Membrane Fusion.

本文引用的文献

1
Development of immunochromatography strip-test using nanocolloidal gold-antibody probe for the rapid detection of aflatoxin B1 in grain and feed samples.使用纳米胶体金-抗体探针开发免疫层析试纸条检测谷物和饲料样品中黄曲霉毒素B1的方法。
J Microbiol Biotechnol. 2007 Oct;17(10):1629-37.
中和单克隆抗体通过阻断膜融合抑制 SARS-CoV-2 感染。
Microbiol Spectr. 2022 Apr 27;10(2):e0181421. doi: 10.1128/spectrum.01814-21. Epub 2022 Mar 16.
4
Development and characterization of mouse monoclonal antibodies targeting to distinct epitopes of Zika virus envelope protein for specific detection of Zika virus.开发和鉴定针对寨卡病毒包膜蛋白不同表位的鼠源单克隆抗体，用于寨卡病毒的特异性检测。
Appl Microbiol Biotechnol. 2021 Jun;105(11):4663-4673. doi: 10.1007/s00253-021-11364-1. Epub 2021 May 27.