College of Agronomy, Northwest A&F University, Yangling, 712100, Shaanxi, China.
Planta. 2021 Mar 26;253(4):83. doi: 10.1007/s00425-021-03601-8.
Bioinformatic analysis identified the function of genes regulating wheat fertility. Barley stripe mosaic virus-induced gene silencing verified that the genes TaMut11 and TaSF3 are involved in pollen development and related to fertility conversion. Environment-sensitive genic male sterility is of vital importance to hybrid vigor in crop production and breeding. Therefore, it is meaningful to study the function of the genes related to pollen development and male sterility, which is still not fully understand currently. In this study, YanZhan 4110S, a new thermo-sensitive genic male sterility wheat line, and its near-isogenic line YanZhan 4110 were analyzed. Through comparative transcriptome basic bioinformatics and weighted gene co-expression network to further identify some hub genes, the genes TaMut11 and TaSF3 associated with pollen development and male sterility induced by high-temperature were identified in YanZhan 4110S. Further verification through barley stripe mosaic virus-induced gene silencing elucidated that the silencing of TaMut11 and TaSF3 caused pollen abortion, finally resulting in the declination of fertility. These findings provided data on the abortive mechanism in environment-sensitive genic male sterility wheat.
生物信息学分析鉴定了调控小麦育性的基因功能。大麦黄花叶病毒诱导的基因沉默验证了 TaMut11 和 TaSF3 基因参与花粉发育，并与育性转换有关。环境敏感型基因雄性不育对作物生产和育种中的杂种优势至关重要。因此，研究与花粉发育和雄性不育相关的基因功能具有重要意义，但目前对此了解还不充分。本研究对新的温敏型基因雄性不育小麦品系烟展 4110S 及其近等基因系烟展 4110 进行了分析。通过比较转录组基础生物信息学和加权基因共表达网络进一步鉴定了一些关键基因，在烟展 4110S 中鉴定到与高温诱导花粉败育和雄性不育相关的 TaMut11 和 TaSF3 基因。通过大麦黄花叶病毒诱导的基因沉默进一步验证表明，TaMut11 和 TaSF3 的沉默导致花粉败育，最终导致育性下降。这些发现为环境敏感型基因雄性不育小麦的败育机制提供了数据。
