Jiao Jian, Wang Yichun, Selvaraj Jonathan Nimal, Xing Fuguo, Liu Yang
Institute of Agro-Products Processing Science and Technology, Chinese Academy of Agricultural Sciences/Key Laboratory of Agro-Products Processing, Ministry of Agriculture, Beijing, P.R. China.
PLoS One. 2015 May 8;10(5):e0126621. doi: 10.1371/journal.pone.0126621. eCollection 2015.
MicroRNAs (miRNAs) play important roles in growth, development, and response to environmental changes in plants. Based on the whole-genome shotgun sequencing strategy, more and more wheat miRNAs have been annotated. Now, there is a need for an effective technology to analyse endogenous miRNAs function in wheat. We report here that the modified barley stripe mosaic virus (BSMV)-induced miRNAs silencing system can be utilized to silence miRNAs in wheat. BSMV-based miRNA silencing system is performed through BSMV-based expression of miRNA target mimics to suppress miR159a and miR3134a. The relative expression levels of mature miR159a and miR3134a decrease with increasing transcript levels of their target genes in wheat plants. In summary, the developed approach is effective in silencing endogenous miRNAs, thereby providing a powerful tool for biological function analyses of miRNA molecules in common wheat.
微小RNA（miRNA）在植物的生长、发育以及对环境变化的响应中发挥着重要作用。基于全基因组鸟枪法测序策略，越来越多的小麦miRNA已得到注释。目前，需要一种有效的技术来分析小麦中内源性miRNA的功能。我们在此报告，改良的大麦条纹花叶病毒（BSMV）诱导的miRNA沉默系统可用于沉默小麦中的miRNA。基于BSMV的miRNA沉默系统是通过基于BSMV表达miRNA靶标模拟物来抑制miR159a和miR3134a实现的。在小麦植株中，成熟miR159a和miR3134a的相对表达水平随着其靶基因转录水平的增加而降低。总之，所开发的方法在沉默内源性miRNA方面是有效的，从而为普通小麦中miRNA分子的生物学功能分析提供了一个强大的工具。
