Genome-Directed Discovery of Tetrahydroisoquinolines from Deep-Sea Derived SCSIO 3406.

A genome-directed discovery strategy to identify new tetrahydroisoquinolines (THIQs) was applied to deep-sea derived SCSIO 3406; 11 THIQs were found representing three THIQ classes. Known aclidinomycins A () and B () were isolated along with nine new compounds, aclidinomycins C-K (-). The structures were elucidated using extensive spectroscopic analyses and single-crystal X-ray diffraction methods. The core skeleton of compounds - contains a fused tetrahydropyran (THP) as an integral part of a distinct type of 6/6/6/6/5/5 polycyclic motif. This is the first report of such a system. Beyond their discovery, we also report here a proposed biosynthetic route to these interesting natural products as well as a preliminary survey of their antimicrobial activities.