Department of Biotechnology, Bu-Ali Sina University, 6517838695, Hamedan, Iran; Department of Chemistry and Bioscience, Aalborg University, Fredrik Bajers Vej 7H, DK-9220, Aalborg East, Denmark.
Department of Biotechnology, Bu-Ali Sina University, 6517838695, Hamedan, Iran.
Environ Pollut. 2021 Jul 1;280:116877. doi: 10.1016/j.envpol.2021.116877. Epub 2021 Mar 9.
Recent studies have indicated that Galleria mellonella larvae ingest polyethylene films and the degradation mechanism could inspire biotechnological exploitation for degrading plastic to eliminate global pollution from plastic waste. In this study, we tested the chemical compositions of masticated and ingested different plastic types by G. mellonella. High throughput sequencing of 16S rRNA gene was used to characterize the alteration of the microbial communities derived from salivary glands, gut contents and whole G. mellonella larvae. Our results indicated that G. mellonella is able to masticate polyethylene (PE), expanded polystyrene (EPS) and polypropylene (PP) and convert it to small particles with very large and chemically modified surfaces. The characteristics of the polymer affect the rate of damage. Formation of functional carbonyl groups on the appearance of oxidized metabolic intermediates of polyolefins in the frass samples observed. We found that the mastication of EPS, PP or PE could significantly alter the microbial composition in the gut content while it did not appear to influence the salivary glands microbial community. Representatives of Desulfovibrio vulgaris and Enterobacter grew with the PE diet while mastication of polystyrene and polypropylene increased the abundance of Enterococcus. The evaluation of bacterial communities in whole larvae confirmed the obtained result and additionally showed that the abundance of Paenibacillus, Corynebacterium and Commamonadaceae increased by Styrofoam (EPS) consumption.
最近的研究表明，桔小实蝇幼虫会摄取聚乙烯薄膜，其降解机制可以启发生物技术的开发，以降解塑料，消除塑料垃圾造成的全球性污染。在这项研究中，我们测试了桔小实蝇幼虫咀嚼和摄取不同塑料类型的化学成分。使用 16S rRNA 基因高通量测序来描述来源于唾液腺、肠道内容物和整个桔小实蝇幼虫的微生物群落的变化。我们的结果表明，桔小实蝇能够咀嚼聚乙烯（PE）、膨胀聚苯乙烯（EPS）和聚丙烯（PP），并将其转化为具有非常大且化学修饰表面的小颗粒。聚合物的特性会影响损伤的速度。在粪便样本中观察到聚烯烃氧化代谢中间产物表面形成功能性羰基基团。我们发现，EPS、PP 或 PE 的咀嚼可以显著改变肠道内容物中的微生物组成，而似乎不会影响唾液腺微生物群落。普通脱硫弧菌和肠杆菌属的代表种随着 PE 饮食而生长，而聚苯乙烯和聚丙烯的咀嚼增加了肠球菌的丰度。对整个幼虫的细菌群落的评估证实了所得结果，并进一步表明，食用泡沫塑料（EPS）增加了不动杆菌属、棒状杆菌属和根瘤菌科的丰度。
