Department of Spine Surgery, Chifeng Municipal Hospital, Chifeng City, Inner Mongolia Autonomous Region, 024000, China.
Department of Trauma Orthopedics, Chifeng Municipal Hospital, Chifeng City, Inner Mongolia Autonomous Region, 024000, China.
Biochem Biophys Res Commun. 2021 May 14;553:126-133. doi: 10.1016/j.bbrc.2021.03.070. Epub 2021 Mar 23.
Circular RNA (circRNA) homeodomain-interacting protein kinase 3 (circ_HIPK3) has recently reported as regulator in spinal cord injury (SCI). The regulatory mechanism of circ_HIPK3 in SCI was further researched in this study. Circ_HIPK3 expression was inhibited by CoCl in AGE1.HN cells. The CoCl-induced cell cycle arrest, cell proliferation inhibition and apoptosis promotion were mitigated by overexpression of circ_HIPK3. Circ_HIPK3 could target miR-222-3p and circ_HIPK3 repressed the CoCl-induced neuronal cell injury by sponging miR-222-3p. DUSP19 was a target gene of miR-222-3p and circ_HIPK3 affected the expression of DUSP19 via binding to miR-222-3p. The regulation of circ_HIPK3 in CoCl-induced injury of AGE1.HN cells was associated with the upregulation of DUSP19. Circ_HIPK3 acted as a pathogenic inhibitor in the progression of SCI via the miR-222-3p-mediated DUSP19 upregulation.
环状 RNA（circRNA）同源结构域相互作用蛋白激酶 3（circ_HIPK3）最近被报道为脊髓损伤（SCI）的调节因子。本研究进一步研究了 circ_HIPK3 在 SCI 中的调节机制。CoCl 在 AGE1.HN 细胞中抑制 circ_HIPK3 的表达。circ_HIPK3 的过表达减轻了 CoCl 诱导的细胞周期停滞、细胞增殖抑制和细胞凋亡促进。circ_HIPK3 可以靶向 miR-222-3p，circ_HIPK3 通过海绵 miR-222-3p 抑制 CoCl 诱导的神经元细胞损伤。DUSP19 是 miR-222-3p 的靶基因，circ_HIPK3 通过与 miR-222-3p 结合影响 DUSP19 的表达。circ_HIPK3 在 CoCl 诱导的 AGE1.HN 细胞损伤中的调节与 DUSP19 的上调有关。circ_HIPK3 通过 miR-222-3p 介导的 DUSP19 上调在 SCI 的进展中充当致病抑制剂。
