Department of Normal Physiology, Danylo Halytsky Lviv National Medical University, Lviv, Ukraine.
Department of Toxicological and Analytical Chemistry, Danylo Halytsky Lviv National Medical University, Lviv, Ukraine.
Drug Metab Pers Ther. 2021 Mar 29;36(3):239-245. doi: 10.1515/dmpt-2020-0187.
Osteoporosis often occurs in individuals of different age groups, frequently during menopause and after ovariectomy. It increases the risk of pathological fractures almost twice. The aim of our research was to assess bone metabolism, nanocomposite structure of the tibia under conditions of ovariectomy and zoledronic acid treatment.
X-ray diffraction has been performed for nanostructure analysis of mineral crystallites and crystal lattice of hydroxyapatite in the tibia samples of ovariectomized rats with additional application of bisphosphonate zoledronic acid (0.025 mg/kg). Markers of remodeling - osteocalcin, alkaline phosphatase, tartrate resistant acid phosphatase 5b - were determined. Quantitative amount of calcium in the bones was detected by atomic absorption method.
Zoledronic acid prevented loss of mineral mass after ovariectomy. Rats after ovariectomy, treated with zoledronic acid, showed statistically higher (р<0.05) values of crystalline phase and calcium content compared with the SHAM-surgery and ovariectomy groups (р<0.05). Zoledronic acid inhibited bone remodeling, which is proved by tartrate resistant acid phosphatase 5b reduction and inhibition of osteoclasts during the experiment.
These results enable to suggest that zoledronic acid can improve mineral mass of the bone during menopause in individuals of different age groups.
骨质疏松症常发生于不同年龄组的个体，常发生于绝经后和卵巢切除术后。它使病理性骨折的风险增加近两倍。我们的研究目的是评估去卵巢和唑来膦酸治疗条件下的骨代谢和胫骨纳米复合材料结构。
对去卵巢大鼠胫骨样本进行了 X 射线衍射，以分析矿物质晶体和羟磷灰石晶格的纳米结构，同时还应用了双膦酸盐唑来膦酸（0.025mg/kg）。测定了骨重建标志物——骨钙素、碱性磷酸酶、抗酒石酸酸性磷酸酶 5b。采用原子吸收法检测骨中钙的定量含量。
唑来膦酸可防止去卵巢后矿物质质量的丢失。与 SHAM 手术和卵巢切除术组相比，用唑来膦酸治疗的去卵巢大鼠的晶相和钙含量具有统计学上更高的（р<0.05）值。唑来膦酸抑制了骨重建，这一点通过抗酒石酸酸性磷酸酶 5b 的减少和实验过程中破骨细胞的抑制得到了证明。
这些结果表明，唑来膦酸可以改善不同年龄组绝经后个体的骨矿物质质量。
