Zhang Yu, Li Tengjie, Xu Mingjie, Guo Jianheng, Zhang Chunmei, Feng Zhaozhong, Peng Xue, Li Zongyun, Xing Ke, Qin Sheng
School of Life Science, the Key Laboratory of Biotechnology for Medicinal Plant of Jiangsu Province, Jiangsu Normal University, Xuzhou 221116, Jiangsu, PR China.
School of Life Science, the Key Laboratory of Biotechnology for Medicinal Plant of Jiangsu Province, Jiangsu Normal University, Xuzhou 221116, Jiangsu, PR China.
Pestic Biochem Physiol. 2021 Mar;173:104777. doi: 10.1016/j.pestbp.2021.104777. Epub 2021 Jan 15.
Ceratocystis fimbriata is the pathogen of black rot disease, which widely exists in sweet potato producing areas all over the world. The antifungal activity of volatile organic compounds (VOCs) released by Pseudomonas chlororaphis subsp. aureofaciens SPS-41 against C. fimbriata was reported in our previous study. In this study, we attempted to reveal the underlying antifungal mechanism of SPS-41 volatiles. Our results showed that the VOCs released by SPS-41 caused the morphological change of hyphae, destroyed the integrity of cell membrane, reduced the content of ergosterol, and induced massive accumulation of reactive oxygen species in C. fimbriata cells. Furthermore, SPS-41 fumigation decreased the mitochondrial membrane potential, acetyl-CoA and pyruvate content of C. fimbriata cells, as well as the mitochondrial dehydrogenases activity. In addition, the VOCs generated by SPS-41 reduced the intracellular ATP content and increased the extracellular ATP content of C. fimbriata. In summary, SPS-41 fumigation exerted its antifungal activity by inducing oxidative stress and mitochondrial dysfunction in C. fimbriata.
甘薯长喙壳菌是甘薯黑腐病的病原菌，广泛存在于世界各地的甘薯产区。我们之前的研究报道了金色产氯假单胞菌亚种SPS-41释放的挥发性有机化合物（VOCs）对甘薯长喙壳菌的抗真菌活性。在本研究中，我们试图揭示SPS-41挥发物潜在的抗真菌机制。我们的结果表明，SPS-41释放的VOCs导致了菌丝形态的变化，破坏了细胞膜的完整性，降低了麦角甾醇的含量，并诱导甘薯长喙壳菌细胞中活性氧的大量积累。此外，SPS-41熏蒸降低了甘薯长喙壳菌细胞的线粒体膜电位、乙酰辅酶A和丙酮酸含量，以及线粒体脱氢酶活性。此外，SPS-41产生的VOCs降低了甘薯长喙壳菌的细胞内ATP含量，增加了其细胞外ATP含量。综上所述，SPS-41熏蒸通过诱导甘薯长喙壳菌的氧化应激和线粒体功能障碍发挥其抗真菌活性。
