Gao Fangyuan, Zhou Xiaosi, Yang Dongjing, Chen Jingwei, Kgosi Veronica Tshegofatso, Zhang Chengling, Ma Jukui, Tang Wei, Liang Zhao, Sun Houjun
Key Laboratory of Biology and Genetic Improvement of Sweetpotato, Xuzhou Institute of Agricultural Sciences in Jiangsu Xuhuai District, Ministry of Agriculture, Xuzhou 221131, China.
Jiangsu Coastal Area Institute of Agricultural Sciences, Yancheng 224002, China.
Genes (Basel). 2024 Nov 28;15(12):1540. doi: 10.3390/genes15121540.
: Sweetpotato black rot, caused by , is a severe fungal disease in sweetpotato production. Biological control strategies represent a promising, environmentally sustainable approach to managing this disease. This study investigates the biocontrol potential of SFB-1 against . : The antagonistic activities of strain SFB-1 on were assessed through assays, including evaluations of mycelial inhibition, spore germination, and mycelial morphology. Pathogenicity assays on harvested sweetpotato roots assessed lesion diameter and depth. A transcriptomic analysis of exposed to strain SFB-1 was performed to explore the underlying antifungal mechanism of SFB-1 on . The qRT-PCR was employed to validate the RNA-seq results. : assays demonstrated that strain SFB-1 inhibited mycelial growth by up to 81.01%, caused mycelial swelling, and completely suppressed spore germination at 10 CFU/mL. The cell-free supernatant of strain SFB-1 also suppressed growth. Pathogenicity assays revealed that strain SFB-1 treatments reduced lesion diameter and depth on harvested sweetpotato roots by over 50% compared to untreated controls. Transcriptomic analysis of treated with strain SFB-1 identified 1164 differentially expressed genes, with significant alterations in genes associated with cell wall integrity, cell membrane stability, spore germination, detoxification, and antioxidant responses. The qRT-PCR validation of 16 genes confirmed the consistency with the RNA-seq results. : SFB-1 demonstrates significant biocontrol efficacy against through multiple mechanisms, positioning it as a promising solution for the sustainable management of sweetpotato black rot.
甘薯黑腐病由[病原菌名称缺失]引起，是甘薯生产中的一种严重真菌病害。生物防治策略是一种有前景的、环境可持续的病害管理方法。本研究调查了SFB - 1对[病原菌名称缺失]的生防潜力。：通过多种测定评估菌株SFB - 1对[病原菌名称缺失]的拮抗活性，包括菌丝抑制、孢子萌发和菌丝形态评估。对收获的甘薯根进行致病性测定，评估病斑直径和深度。对暴露于菌株SFB - 1的[病原菌名称缺失]进行转录组分析，以探索SFB - 1对[病原菌名称缺失]的潜在抗真菌机制。采用qRT - PCR验证RNA测序结果。：测定表明，菌株SFB - 1在10 CFU/mL时可抑制[病原菌名称缺失]菌丝生长达81.01%，导致菌丝肿胀，并完全抑制孢子萌发。菌株SFB - 1的无细胞上清液也抑制[病原菌名称缺失]生长。致病性测定显示，与未处理对照相比，菌株SFB - 1处理使收获的甘薯根上的病斑直径和深度减少了50%以上。对用菌株SFB - 1处理的[病原菌名称缺失]进行转录组分析，鉴定出1164个差异表达基因，与细胞壁完整性、细胞膜稳定性、孢子萌发、解毒和抗氧化反应相关的基因有显著变化。对16个基因的qRT - PCR验证证实了与RNA测序结果的一致性。：SFB - 1通过多种机制对[病原菌名称缺失]表现出显著的生防效果，使其成为甘薯黑腐病可持续管理的有前景的解决方案。
