Laboratory of Plant Extractives, Department of Industrial Pharmacy, Federal University of Santa Maria, RS, Brazil.
Department of Physiology and Pharmacology, Federal University of Santa Maria, RS, Brazil.
Microb Pathog. 2021 May;154:104871. doi: 10.1016/j.micpath.2021.104871. Epub 2021 Mar 24.
This study points to evaluate the effects of pre-treatment with standardized dry extract of Curcuma longa (Motore™) added to the diet (0; 250; 500; and 750 mg/kg) on oxidative stress parameters, longevity, and therapeutic success in Rhamdia quelen experimentally infected with Aeromonas hydrophila (MF 372510). After treatment, the liver and kidney were collected to determine non-enzymatic oxidative parameters such as the formation of thiobarbituric acid reactive substances (TBARS), non-protein thiols (NPSH), and quantification of reactive oxygen species (ROS) levels. Also, two enzymatic antioxidant parameters were evaluated: superoxide dismutase (SOD) and catalase (CAT) activities. The results showed an increase of ROS and TBARS levels, a depletion in NPSH, and a decrease of SOD and CAT activities in infected fish compared to control. The highest Motore™ dose minimized the deleterious effect of A. hydrophila infection improving longevity, oxidative status, and survival rate. The addition of 750 mg Motore™/kg feed is recommended for silver catfish in fish farming. Serious economic losses in Rhamdia quelen culture caused by Aeromonas hydrophila infections can be prevented by the addition of Motore™ to the diet.
本研究旨在评估在饮食中添加标准化干姜黄提取物（Motore™）预处理（0；250；500；和 750mg/kg）对实验感染嗜水气单胞菌（MF 372510）的圆口铜鱼的氧化应激参数、寿命和治疗成功率的影响。治疗后，收集肝脏和肾脏以确定非酶促氧化参数，如硫代巴比妥酸反应物质（TBARS）的形成、非蛋白巯基（NPSH）和活性氧（ROS）水平的定量。此外，还评估了两种酶促抗氧化参数：超氧化物歧化酶（SOD）和过氧化氢酶（CAT）活性。结果表明，与对照组相比，感染鱼体内的 ROS 和 TBARS 水平升高，NPSH 耗竭，SOD 和 CAT 活性降低。最高剂量的 Motore™ 最小化了嗜水气单胞菌感染的有害影响，提高了寿命、氧化状态和存活率。建议在养殖银鲳的饲料中添加 750mg/kg 的 Motore™。通过在饮食中添加 Motore™ 可以预防由嗜水气单胞菌感染引起的圆口铜鱼养殖中的严重经济损失。
