Dihl F, Bégeot M, Loevenhruck C, Dubois M P, Dubois P M
CNRS UA 559, Faculté de Médecine Lyon-Sud, Oullins, France.
Anat Embryol (Berl). 1988;178(1):21-7. doi: 10.1007/BF00305010.
The fetal period constitutes a determinant stage in the ontogenesis of the hypothalamo-hypophysial-gonadal system. This work mainly concerns gonadotropic and thyrotropic functions and compares their different aspects in two strains of fetal mice. Balb/c and C57 BL6 fetal mice were studied at 16, 17, 18 and 19 days of gestation. The appearance and distribution of immunoreactive gonadotropic and thyrotropic cells of the anterior pituitary were observed by immunocytology using an indirect method with anti-porcine luteinizing hormone beta serum, anti-bovine thyrotrope hormone serum after saturation with bovine luteinizing hormone and anti-rat luteinizing hormone serum. In the two strains and in both sexes, LH gonadotropes appeared at 17 days of gestation and preferentially localized in the ventral part of the anterior lobe; a similar distribution was noted at 18 days and there was an increase in the number and staining intensity of labeled cells. By 19 days of gestation the gonadotrophs seemed more numerous, more generally distributed throughout the gland and often abutted to sinusoidal capillaries. An account of immunoreactive cells with anti-porcine luteinizing hormone serum and statistical evaluation of the results performed by variance analysis showed significant differences between the two strains. LH gonadotropic cells were always more numerous at each day of gestation in Balb/c fetuses especially in female fetuses. The possibility of a different evolution and/or differentiation for this cell population is discussed. Comparison of gonadotropic function between rat fetuses, mouse fetuses and human fetuses lead us to conclude that mouse fetuses appeared as an experimental model more closely related to human fetuses.
胎儿期是下丘脑 - 垂体 - 性腺系统个体发育中的一个决定性阶段。这项工作主要关注促性腺和促甲状腺功能，并比较了两种品系胎儿小鼠的不同方面。对妊娠16、17、18和19天的Balb/c和C57 BL6胎儿小鼠进行了研究。使用间接方法，用抗猪促黄体生成素β血清、用牛促黄体生成素饱和后的抗牛促甲状腺激素血清以及抗大鼠促黄体生成素血清，通过免疫细胞学法观察垂体前叶免疫反应性促性腺和促甲状腺细胞的出现和分布。在这两个品系和两种性别中，促黄体生成素促性腺细胞在妊娠17天时出现，优先定位于前叶腹侧部分；在18天时观察到类似的分布，标记细胞的数量和染色强度有所增加。到妊娠19天时，促性腺细胞似乎更多，更广泛地分布于整个腺体，并且常常紧邻窦状毛细血管。用抗猪促黄体生成素血清对免疫反应性细胞进行计数，并通过方差分析对结果进行统计学评估，结果显示两个品系之间存在显著差异。在妊娠的每一天，Balb/c胎儿尤其是雌性胎儿中的促黄体生成素促性腺细胞总是更多。讨论了该细胞群体不同进化和/或分化的可能性。对大鼠胎儿、小鼠胎儿和人类胎儿的促性腺功能进行比较后，我们得出结论，小鼠胎儿似乎是与人类胎儿关系更密切的实验模型。
