Bergren C T, Bodzin J H, Cortez J A
Department of Surgery, Mount Carmel Mercy Hospital, Detroit, Michigan 48235.
Am Surg. 1988 Jun;54(6):333-6.
A rat model was developed to determine the efficacy of oxygen free radical scavenger compounds in improving small bowel anastomotic healing in ischemia. 50 Sprague-Dawley rats underwent laparotomy and were divided into groups: I. sham operation; II. ischemia produced by ligation of mesenteric vessels along 3-5 cm of bowel; III. bowel transection and anastomosis; IV. ligation of vessels with bowel transection and anastomosis; V. ligation of vessels, bowel transection and IV administration of superoxide dismutase (SOD) (5000 U/kg) prior to anastomosis. All surviving animals were sacrificed after 2 weeks. Anastomotic tensile strength and histology were evaluated. Percent survival and the average length of survival for all groups is seen in the table below. (table: see text) A significant decrease in survival was present with the anastomotic group and the ischemic anastomotic group when compared with controls. An improved survival similar to ischemia alone was present in SOD group. No significant difference was noted between SOD and control groups. The results of this study indicate an improved survival rate and length of survival similar to controls in animals undergoing ischemic and penetrating injury to the bowel with the use of oxygen free radical scavenger compounds prior to anastomosis.
建立了一个大鼠模型，以确定氧自由基清除剂化合物在改善缺血状态下小肠吻合口愈合方面的疗效。50只Sprague-Dawley大鼠接受剖腹手术，并被分为以下几组：I. 假手术组；II. 通过结扎3-5厘米肠段的肠系膜血管产生缺血组；III. 肠切断吻合组；IV. 血管结扎伴肠切断吻合组；V. 血管结扎、肠切断并在吻合术前静脉注射超氧化物歧化酶（SOD）（5000 U/kg）组。所有存活的动物在2周后被处死。评估吻合口抗张强度和组织学情况。下表列出了所有组别的存活率百分比和平均存活时间。（表格：见正文）与对照组相比，吻合组和缺血吻合组的存活率显著降低。SOD组的存活率有所提高，与单纯缺血组相似。SOD组和对照组之间未观察到显著差异。本研究结果表明，在吻合术前使用氧自由基清除剂化合物的情况下，遭受肠缺血和穿透性损伤的动物的存活率和存活时间有所提高，与对照组相似。
