Laboratory of Food Chemistry and Nutrition Science, Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Key Laboratory of Agro-Products Processing, Ministry of Agriculture and Rural Affairs, No. 2 Yuan Ming Yuan West Road, Haidian District, PO Box 5109, Beijing 100193, China.
Laboratory of Food Chemistry and Nutrition Science, Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Key Laboratory of Agro-Products Processing, Ministry of Agriculture and Rural Affairs, No. 2 Yuan Ming Yuan West Road, Haidian District, PO Box 5109, Beijing 100193, China.
Ultrason Sonochem. 2021 May;73:105528. doi: 10.1016/j.ultsonch.2021.105528. Epub 2021 Mar 15.
Effects of ultrasound (US, 300, 400, and 500 W) and slightly acidic electrolyzed water (SAEW, 10, 30, and 50 mg/L) combination on inactivating Rhizopus stolonifer in sweet potato tuberous roots (TRs) were investigated. US at 300, 400, and 500 W simultaneous SAEW with available chlorine concentration of 50 mg/L at 40 and 55 °C for 10 min significantly inhibited colony diameters (from 90.00 to 6.00-71.62 mm) and spores germination (p < 0.05). US + SAEW treatment could destroy cell membrane integrity and lead to the leakage of nucleic acids and proteins (p < 0.05). Scanning and transmission electron microscopy results showed that US + SAEW treatment could damage ultrastructure of R. stolonifer, resulted in severe cell-wall pitting, completely disrupted into debris, apparent separation of plasma wall, massive vacuoles space, and indistinct intracellular organelles. US500 + SAEW50 treatment at 40 and 55 °C increased cell membrane permeability, and decreased mitochondrial membrane potential of R. stolonifer. In addition, US500 + SAEW50 at 40 °C and US300 + SAEW50 at 55 °C controlled R. stolonifer growth in sweet potato TRs during 20 days of storage, suggesting effective inhibition on the infection of R. stolonifer. Therefore, US + SAEW treatment could be a new efficient alternative method for storing and preserving sweet potato TRs.
研究了超声（US，300、400 和 500 W）和微酸性电解水（SAEW，10、30 和 50 mg/L）联合处理对抑制甘薯块根根腐病菌（Rhizopus stolonifer）的影响。在 40 和 55°C 下，300、400 和 500 W 的 US 与有效氯浓度为 50 mg/L 的 SAEW 同时作用 10 min，可显著抑制菌落直径（从 90.00 减小至 6.00-71.62 mm）和孢子萌发（p<0.05）。US+SAEW 处理可破坏细胞膜完整性，导致核酸和蛋白质渗漏（p<0.05）。扫描和透射电子显微镜结果表明，US+SAEW 处理可破坏根腐病菌的超微结构，导致细胞壁严重凹陷，完全破碎成碎片，明显分离出质膜，大量空泡空间，以及不明显的细胞内细胞器。在 40 和 55°C 下，US500+SAEW50 处理增加了细胞膜通透性，降低了根腐病菌的线粒体膜电位。此外，在 40°C 下 US500+SAEW50 和在 55°C 下 US300+SAEW50 可控制甘薯块根在 20 天贮藏期内根腐病菌的生长，表明对根腐病菌的感染有有效抑制作用。因此，US+SAEW 处理可能是一种新的有效替代方法，用于甘薯块根的贮藏和保鲜。
