Department of Chemistry, Facultad de Ciencias, Universidad de Burgos, Plaza de Misael Bañuelos s/n, 09001 Burgos, Spain.
Department of Biotechnology and Food Science, Universidad de Burgos, Plaza de Misael Bañuelos s/n, 09001 Burgos, Spain.
Food Chem. 2021 Sep 1;355:129629. doi: 10.1016/j.foodchem.2021.129629. Epub 2021 Mar 18.
A new original application for a polyacrylic film based on the monomers 2-(dimethylamino)ethyl methacrylate (NNDA), 2-hydroxyethyl acrylate (2HEA) and methylmethacrylate (MMA) as a starch azure container has been set up for a simple determination of honey diastase activity. The proposed method is based on the correlation of reducing sugars generated during the enzymatic process with the Schade reference assay. The polyacrylic film is charged with starch azure acting as a container for this substance; thus, the starch does not interfere in the measurement of reducing sugars, so that the diastase activity is easily calculated. The method has been contrasted with Schade method, showing good correlation and differences under 0.4% between methods in some honey samples. The polyacrylic film has great potential for the routine honey diastase activity assessment in small laboratories, dramatically reducing analysis time and cost.
一种基于 2-（二甲氨基）乙基甲基丙烯酸酯（NNDA）、2-羟基乙基丙烯酸酯（2HEA）和甲基丙烯酸甲酯（MMA）单体的新型聚丙烯酸酯薄膜的原始应用被提出，作为一种简单测定蜂蜜淀粉酶活性的淀粉蓝容器。该方法基于酶促过程中生成的还原糖与 Schade 参考测定法的相关性。聚丙烯酸酯薄膜带有作为该物质容器的淀粉蓝；因此，淀粉不会干扰还原糖的测量，从而可以轻松计算淀粉酶活性。该方法与 Schade 方法进行了对比，在一些蜂蜜样品中，两种方法的相关性良好，差异在 0.4%以内。对于小型实验室的常规蜂蜜淀粉酶活性评估，聚丙烯酸酯薄膜具有很大的潜力，可以大大缩短分析时间和降低成本。
