Ghanta V K, Miura T, Hiramoto N S, Hiramoto R N
Department of Biology, University of Alabama, Birmingham 35294.
Ann N Y Acad Sci. 1988;521:29-42. doi: 10.1111/j.1749-6632.1988.tb35263.x.
We have reported the effect of classical (Pavlovian) conditioning of natural immunity on survival of tumor-bearing mice. In the first study, we have observed that mice conditioned, transplanted with tumor, and re-exposed to conditioned stimulus (camphor odor) had an increase in median survival (day 43, as compared to days 34, 38, and 37 of various control groups). Two of these conditioned mice lived more than 120 days and showed early tumor growth, but were free of disease at day 97. We report the observations of a repeat study. Two groups of conditioned mice were used for these studies. One group was re-exposed to the conditioning stimulus following transplantation with tumor (CND) and the second group was not re-exposed to odor of camphor (CNDo). Statistically significant delay in growth of MOPC 104E in the CND group was observed when compared with the CNDo group. The survival data supports the observations of tumor IgM values. In an independent study, we investigated the possible mechanisms of MOPC 104E regulation in vitro. Plastic adherent spleen cells (macrophage cells) from mice primed in vivo with MOPC 104E tumor cells suppressed tumor IgM production by MOPC cells by 98% and also reduced colony formation by MOPC cells. The possible mechanism(s) of regulation of tumor growth in conditioned mice might be mediated by plastic adherent activated macrophages.
我们已经报道了自然免疫的经典（巴甫洛夫式）条件作用对荷瘤小鼠生存的影响。在第一项研究中，我们观察到经条件作用、移植肿瘤并再次暴露于条件刺激（樟脑气味）的小鼠中位生存期有所延长（第43天，相比之下，各对照组的生存期分别为第34天、38天和37天）。其中两只经条件作用的小鼠存活超过120天，早期有肿瘤生长，但在第97天时无疾病。我们报告重复研究的观察结果。两组经条件作用的小鼠用于这些研究。一组在移植肿瘤后再次暴露于条件刺激（CND），第二组未再次暴露于樟脑气味（CNDo）。与CNDo组相比，观察到CND组中MOPC 104E的生长有统计学意义的延迟。生存数据支持肿瘤IgM值的观察结果。在一项独立研究中，我们在体外研究了MOPC 104E调节的可能机制。体内用MOPC 104E肿瘤细胞致敏的小鼠的塑料贴壁脾细胞（巨噬细胞）将MOPC细胞的肿瘤IgM产生抑制了98%，并减少了MOPC细胞的集落形成。条件作用小鼠中肿瘤生长调节的可能机制可能由塑料贴壁活化巨噬细胞介导。
