Institute of Food Science and Technology, National Taiwan University, Taiwan; Department of Nutrition, China Medical University, Taichung, Taiwan.
Institute of Food Science and Technology, National Taiwan University, Taiwan.
Food Res Int. 2021 Apr;142:110143. doi: 10.1016/j.foodres.2021.110143. Epub 2021 Jan 20.
Obesity is related to energy imbalance and energy metabolism. In this study, we investigated the anti-obesity effects of Garcinia indica extract (GIE), Coleus forskohlii extract (CFE), and the combinations of these two extracts in a 3T3-L1 cells and high-fat diet (HFD)-induced obese mice. In vitro, GIE showed better effect on TG content than CFE, CFE showed better effect on glycerol released than GIE, and the combinations of GIE and CFE showed both effects compared with GIE and CFE alone. In vivo, GIE, LMIX (0.005% GIE + 0.025% CFE), and HMIX (0.01% GIE + 0.025% CFE) down-regulated adipogenesis-related transcription factors PPARγ and C/EBPα protein expression, CFE promoted lipolysis by up-regulated p-HSL and p-PKA protein expression, and four supplementations promoted fatty acid β-oxidation by up-regulating CPT-1A and PPARα protein expression to decrease lipid accumulation in adipose tissue. Moreover, we found that CFE, LMIX and HMIX, except GIE exert increasing the abundance of Bacteroides caccae compared with HFD group. Overall, GIE, CFE, and the combinations of GIE and CFE were able to decrease body weight and adipocyte size by promoting fatty acid β-oxidation and modulating gut microbiota in HFD-induced obese mice.
肥胖与能量失衡和能量代谢有关。在这项研究中，我们研究了藤黄果提取物（GIE）、毛喉鞘蕊花提取物（CFE）及其组合在 3T3-L1 细胞和高脂肪饮食（HFD）诱导肥胖小鼠中的抗肥胖作用。体外实验表明，GIE 对 TG 含量的影响优于 CFE，CFE 对甘油释放的影响优于 GIE，GIE 和 CFE 的组合与 GIE 和 CFE 单独使用相比具有这两种作用。体内实验表明，GIE、LMIX（0.005% GIE+0.025% CFE）和 HMIX（0.01% GIE+0.025% CFE）下调了脂肪生成相关转录因子 PPARγ 和 C/EBPα 蛋白的表达，CFE 通过上调 p-HSL 和 p-PKA 蛋白的表达促进脂肪分解，四种补充剂通过上调 CPT-1A 和 PPARα 蛋白的表达促进脂肪酸β氧化，从而减少脂肪组织中的脂质积累。此外，我们发现 CFE、LMIX 和 HMIX，除 GIE 外，与 HFD 组相比，增加了拟杆菌属的丰度。总体而言，GIE、CFE 及其组合能够通过促进脂肪酸β氧化和调节 HFD 诱导肥胖小鼠的肠道微生物群来降低体重和脂肪细胞大小。
