Wang J T, Danton O, Treyer A, Hamburger M
Pharmaceutical Biology, Department of Pharmaceutical Sciences, University of Basel, Klingelbergstrasse 50, CH-4056 Basel, Switzerland.
Talanta. 2021 Jun 1;228:122216. doi: 10.1016/j.talanta.2021.122216. Epub 2021 Feb 18.
Recent research showed that plant secondary metabolites in pollen may exacerbate the protein-mediated allergic reaction in pollen allergy. It was found that allergenic pollen from various plant families contain significant amounts of electrophiles which may covalently bind to nucleophilic groups of proteins, such as thiol moieties. Electrophiles in pollen of the Asteraceae species are typically sesquiterpene lactones, but the nature of electrophilic metabolites in allergenic pollen of other plant families is unknown. We developed a solid-supported cysteinyl probe in order to selectively extract physiologically relevant electrophiles from pollen extracts, and to enable their subsequent characterization by on-line and off-line spectroscopic analysis. The validity of this approach was evaluated with a selection of structurally different model compounds and with a spiked model extract.
最近的研究表明，花粉中的植物次生代谢产物可能会加剧花粉过敏中蛋白质介导的过敏反应。研究发现，来自不同植物科的致敏花粉含有大量亲电试剂，这些亲电试剂可能会与蛋白质的亲核基团（如硫醇部分）共价结合。菊科植物花粉中的亲电试剂通常是倍半萜内酯，但其他植物科致敏花粉中亲电代谢产物的性质尚不清楚。我们开发了一种固相支持的半胱氨酰探针，以便从花粉提取物中选择性提取生理相关的亲电试剂，并通过在线和离线光谱分析对其进行后续表征。该方法的有效性通过一系列结构不同的模型化合物和加标模型提取物进行了评估。
