Foshan Green Intelligent Manufacturing Research Institute of Xiangtan University, Key Laboratory for Green Organic Synthesis and Application of Hunan Province, Key Laboratory of Environmentally Friendly Chemistry and Application of Ministry of Education, College of Chemistry, Xiangtan University, Xiangtan, 411105, China.
Foshan Green Intelligent Manufacturing Research Institute of Xiangtan University, Key Laboratory for Green Organic Synthesis and Application of Hunan Province, Key Laboratory of Environmentally Friendly Chemistry and Application of Ministry of Education, College of Chemistry, Xiangtan University, Xiangtan, 411105, China; School of Material and Chemical Engineering, Hunan Institute of Technology, Hengyang, 421002, China.
Talanta. 2021 Jun 1;228:122199. doi: 10.1016/j.talanta.2021.122199. Epub 2021 Feb 8.
Herein, a metal-organic framework (UiO-66-NH) with two functions (intrinsic fluorescence and fluorescence quenching ability) is designed to establish a ratiometric fluorescent platform for high-performance miRNA detection. The use of a fluorescent organic ligand endows the MOF material with a strong intrinsic fluorescence at 440 nm. In the presence of target miRNA, the fluorescence signal of the FAM is restored with the triple helix molecular beacons bind to the target. Using the I/I signal as the output, the prepared ratiometric probe was able to eliminate disturbance caused by the sensing environment. Under the optimal reaction conditions, including buffer pH of 7.4, temperature of 37 °C, and response time of 1 h, the best detection results can be obtained. The ratiometric fluorescence probe presented showed good sensitivity and selectivity for detecting miRNA-203 and the limit of detection was 400 pM with a wide linear range from 1 nM to 160 nM. In addition, this method was applied to diluted human serum and cell lysates, and good detection effect was realized.
本文设计了一种具有两种功能（固有荧光和荧光猝灭能力）的金属-有机骨架（UiO-66-NH），建立了一种用于高性能 miRNA 检测的比率荧光平台。荧光有机配体的使用赋予了 MOF 材料在 440nm 处具有很强的固有荧光。在存在靶 miRNA 的情况下，荧光团 FAM 的荧光信号随着三螺旋分子信标与靶标结合而恢复。使用 I/I 信号作为输出，制备的比率探针能够消除传感环境引起的干扰。在最佳反应条件下，包括缓冲液 pH 值为 7.4、温度为 37°C 和响应时间为 1 小时，可以获得最佳的检测结果。所提出的比率荧光探针在检测 miRNA-203 时表现出良好的灵敏度和选择性，检测限为 400pM，线性范围从 1nM 到 160nM。此外，该方法还应用于稀释的人血清和细胞裂解物，并实现了良好的检测效果。
