AgPO NP@MoS nanosheet enhanced F, S-doped BN quantum dot electrochemiluminescence biosensor for K-ras tumor gene detection.

In this research, a novel AgPO NPs@MoS nanosheet-based electrochemiluminescence (ECL) sensing system was developed to provide an effective method for tumor gene detection. At first, fluorine, sulfur-doped BN quantum dot (F, S-BN QD) were prepared as ECL emitter. Sulfur dopant can provide more reactive sites in the ECL reaction. Fluorine atoms in the QD structure further improved the stability of the crystal. Furthermore, AgPO NP@MoS nanosheets were fabricated via a hydrothermal route as ECL reaction catalyst. On the one hand, AgPO NP@MoS nanosheets promoted the generation of more oxidant of coreactant in the F, S-BN QD/HO coreactant ECL pathway. On the other hand, the excellent conductivity of AgPO NP@MoS nanosheets facilitated the electron transfer and effectively reduce the damage of F, S-BN QD by excessive hot electrons. Finally, the proposed biosensor was designed to accurately quantify K-ras tumor gene from 10 fM to 100 pM with a limit of detection (LOD) of 0.2 fM. The sensing system was used to detect K-ras gene in human colorectal cancer tumor and tumor-adjacent tissues samples with satisfactory results. The amplified ECL sensing strategy with AgPO NPs@MoS nanosheet has significant potential value in the clinical detection.