Department of Laboratory Medicine Center, China-Japan Union Hospital of Jilin University, Changchun, 130033, China.
Department of Analytical Chemistry, College of Chemistry, Jilin University, Changchun, 130012, China.
Talanta. 2022 Jan 15;237:122969. doi: 10.1016/j.talanta.2021.122969. Epub 2021 Oct 13.
In this work, a novel electrochemiluminescence (ECL) sensor has been developed to detect miRNA-210 in the serum of triple negative breast cancer (TNBC) patients. The luminous MoS nanosheets were synthesized via the solvothermal method and served as ECL emitters for the first time. As a result, the ECL properties of as-prepared MoS nanosheets were significantly improved. Furthermore, the biomimetic magnetic vesicles were used as capture platform in the ECL sensing strategy. Due to the highly efficient fluidity and magnetic property, the biomimetic vesicles with hairpin aptamers can capture target gene in the serum. After magnetic separation, the captured miRNA-210 can trigger the target-catalyzed hairpin assembly (CHA) sensing process on the magnetic electrode and hybridize MoS nanosheets labeled probe DNA. The concentration of miRNA-210 can be quantified by the ECL enhancement of the MoS nanosheets. This approach has achieved the sensitive detection for miRNA-210 in a range from 1 fM to 100 pM with the detection limit of 0.3 fM. The luminous MoS nanosheets-based ECL sensing system with the biomimetic vesicles would provide a new pathway to explore 2D nanomaterials for developing a wide range of bioanalytical applications.
在这项工作中，开发了一种新型的电化学发光（ECL）传感器来检测三阴性乳腺癌（TNBC）患者血清中的 miRNA-210。通过溶剂热法合成了发光的 MoS 纳米片，并首次将其用作 ECL 发射器。结果，所制备的 MoS 纳米片的 ECL 性能得到了显著提高。此外，仿生磁性囊泡被用作 ECL 传感策略中的捕获平台。由于具有高效的流动性和磁性，带有发夹适体的仿生囊泡可以在血清中捕获靶基因。通过磁分离后，捕获的 miRNA-210 可以在磁性电极上触发靶催化的发夹组装（CHA）传感过程，并与标记有 MoS 纳米片的探针 DNA 杂交。通过 MoS 纳米片的 ECL 增强可以定量检测 miRNA-210，其检测限为 0.3 fM。基于发光 MoS 纳米片的 ECL 传感系统与仿生囊泡相结合，为探索二维纳米材料以开发广泛的生物分析应用提供了新途径。
