Ginel Pedro J, Negrini Joao, Guerra Rafael, Lucena Rosario, Ruiz-Campillo María T, Mozos Elena
Department of Animal Medicine & Surgery, Faculty of Veterinary Medicine, University of Córdoba, 14071 Córdoba, Spain.
Department of Anatomy & Comparative Pathology, Faculty of Veterinary Medicine, University of Córdoba, 14071 Córdoba, Spain.
J Vet Sci. 2021 Mar;22(2):e27. doi: 10.4142/jvs.2021.22.e27.
Ozone is an antimicrobial agent that in experimental and case-control studies has been found to exert a positive effect on wound healing. Wild and pet chelonians frequently present insidious wounds exhibiting secondary infections and/or delayed healing.
Evaluate the effects of topical ozonated sunflower oil on second-intention healing of acute experimental skin wounds in red-eared sliders ().
Randomised within-subject controlled study; Group 1 (n = 24) was used to assess clinical healing features; Group 2 (n = 12) was used for histological evaluation in which two sets of wounds were biopsied at 2, 7, 14, 21, 28 and 42 days over the course of the cicatrisation process. A single 6 mm diameter wound was made on each rear limb and topical ozonated (950 peroxide value) and non-ozonated sunflower oil were applied daily for one week on treated and contralateral control wounds, respectively.
Mean wound size was significantly lower in the ozone-treated group at day 28 ( < 0.0001) with differences of clinical relevance (74.04% vs. 93.05% reduction of initial wound size). Histologically, the acute inflammatory reaction was enhanced in treated wounds, with significantly higher numbers of heterophils ( = 0.0016), lymphocytes ( < 0.001) and fibroblasts ( < 0.001).
Daily topical application of ozonated sunflower oil over the course of one week improved the healing of acute, full-thickness skin wounds in chelonians. This clinical outcome was histologically correlated with an enhanced acute inflammatory reaction, as well as the production and remodelling of collagen fibres.
臭氧是一种抗菌剂，在实验和病例对照研究中已发现其对伤口愈合有积极作用。野生和宠物龟类经常出现隐匿性伤口，伴有继发性感染和/或愈合延迟。
评估局部应用臭氧处理的向日葵油对红耳龟急性实验性皮肤伤口二期愈合的影响。
随机自身对照研究；第1组（n = 24）用于评估临床愈合特征；第2组（n = 12）用于组织学评估，在瘢痕形成过程中于第2、7、14、21、28和42天对两组伤口进行活检。在每个后肢制作一个直径6 mm的伤口，分别在处理的伤口和对侧对照伤口上每天局部应用臭氧处理（过氧化值950）的向日葵油和未处理的向日葵油，持续一周。
在第28天，臭氧处理组的平均伤口大小显著更小（< 0.0001），具有临床相关性差异（初始伤口大小减少74.04% 对 93.05%）。组织学上，处理的伤口中急性炎症反应增强，异嗜性粒细胞（ = 0.0016）、淋巴细胞（< 0.001）和成纤维细胞（< 0.001）数量显著更多。
在一周时间内每天局部应用臭氧处理的向日葵油可改善龟类急性全层皮肤伤口的愈合。这一临床结果在组织学上与急性炎症反应增强以及胶原纤维的产生和重塑相关。
