Dipartimento di Scienze Biomediche, Università degli Studi di Siena, Siena, Italy.
Wound Repair Regen. 2011 Jan-Feb;19(1):107-15. doi: 10.1111/j.1524-475X.2010.00649.x. Epub 2010 Dec 6.
Ozone is well recognized as a bactericidal agent and its beneficial effect on wound healing could be a consequence of this property. Because ozone itself does not penetrate the cells but immediately reacts with polyunsaturated fatty acids, its effects should be the results of oxidative reaction. For this reason, ozonated oils could be a way to deliver ozone messengers to the skin. This paper evaluated the therapeutic effects of three different grades of ozonated sesame oil in acute cutaneous wounds made in the skin of SKH1 mice. Specifically, wound closure rate, histological parameters, and the level of key proteins such as vascular endothelial growth factors and cyclin D1 have been analyzed in relation to the peroxide level present in the ozonated oil. Treatment with moderately ozonated sesame oil--expressed as peroxide value about 1,500)--has a faster wound closure rate in the first 7 days than treatment with oil containing either lower or higher peroxide value, and even with controls. Moreover, under the same treatment, an earlier and higher response of cells involved in wound repair, a higher angiogenesis, as well as an enhanced vascular endothelial growth factors and cyclin D1 expression were observed. The present study shows the validity of ozonated sesame oil in cutaneous wound healing and emphasizes the importance of the ozonation grade.
臭氧作为一种杀菌剂已被广泛认可，其对伤口愈合的有益作用可能是由于这一特性。由于臭氧本身不能穿透细胞，而是立即与多不饱和脂肪酸发生反应，因此其作用应该是氧化反应的结果。出于这个原因，臭氧油可以成为将臭氧信使传递到皮肤的一种方式。本文评估了三种不同等级的臭氧芝麻油在 SKH1 小鼠皮肤急性创面中的治疗效果。具体来说，分析了与臭氧油中过氧化物水平相关的伤口闭合率、组织学参数以及关键蛋白（如血管内皮生长因子和细胞周期蛋白 D1）的水平。与含有较低或较高过氧化物值的油相比，在最初的 7 天内，中臭氧芝麻油（以过氧化物值约 1500 表示）处理具有更快的伤口闭合率，甚至与对照组相比也是如此。此外，在相同的治疗下，参与伤口修复的细胞更早、更高地反应，更高的血管生成，以及增强的血管内皮生长因子和细胞周期蛋白 D1 的表达。本研究表明臭氧芝麻油在皮肤伤口愈合中的有效性，并强调了臭氧处理等级的重要性。
