Bahria university Karachi, Pakistan.
Department of Anatomy, University of Health Sciences, Lahore, Pakistan.
J Ayub Med Coll Abbottabad. 2021 Jan-Mar;33(1):134-138.
The adrenal gland is one of the major endocrine glands involved not only in different physiological functions, but also in response to stress This study was done to asses the effects of L-arginine and insulin on streptozotocin (STZ) induced adrenal gland damage in albino rats.
This laboratory based experimental study on animals was undertaken in the Anatomy department of Basic Medical Sciences Institute (BMSI), Jinnah postgraduate Medical Center (JPMC), Karachi, from February to March 2018. Forty adult, healthy male albino rats were placed into 4 groups (10 each). Group A was taken as control. Group B was given STZ. Group C and D were given STZ as in group B with insulin and L-arginine respectively. Absolute and relative weight of adrenal glands was measured at the end of the study. Tissues from adrenal glands were processed and stained with Haematoxylin and Eosin for the morphometric study.
The absolute as well as relative adrenal weight of animals was significantly raised in group B in comparison to control, although showed a significant recovery in group C and D animals when insulin and L-arginine were added to STZ. Haematoxylin and Eosin (H&E) stained sections of adrenal cortex of STZ treated Group B showed reduced width of zona glomerulosa, with increased width of zona fasciculata and zona reticularis when compared to control. The width of these zones of adrenal cortex recovered to a significant extent when group C and D tissue sections were compared with STZ- treated group B tissue sections.
This study highlighted the protective effect of L-arginine on adrenal gland weight and histology in streptozotocin induced adrenal gland damage, which was comparable to insulin.
肾上腺是主要的内分泌腺之一，不仅参与多种生理功能，还参与应激反应。本研究旨在评估 L-精氨酸和胰岛素对链脲佐菌素（STZ）诱导的白化大鼠肾上腺损伤的影响。
本实验室基于动物的实验研究于 2018 年 2 月至 3 月在卡拉奇吉纳研究生医学中心（JPMC）基础医学科学研究所（BMSI）解剖系进行。将 40 只成年健康雄性白化大鼠分为 4 组（每组 10 只）。A 组为对照组。B 组给予 STZ。C 组和 D 组与 B 组一样给予 STZ，同时分别给予胰岛素和 L-精氨酸。研究结束时测量肾上腺的绝对和相对重量。对肾上腺组织进行处理并进行苏木精和伊红染色进行形态计量学研究。
与对照组相比，B 组动物的肾上腺绝对和相对重量显著升高，但当向 STZ 中添加胰岛素和 L-精氨酸时，C 组和 D 组动物的重量显著恢复。与对照组相比，用 STZ 处理的 B 组动物的肾上腺皮质的苏木精和伊红（H&E）染色切片显示肾小球带宽度减小，束状带和网状带宽度增加。当将 C 组和 D 组的组织切片与 STZ 处理的 B 组的组织切片进行比较时，这些肾上腺皮质带的宽度恢复到显著程度。
本研究强调了 L-精氨酸对链脲佐菌素诱导的肾上腺损伤中肾上腺重量和组织学的保护作用，其作用可与胰岛素相媲美。
