The N.1 Institute for Health, National University of Singapore, Singapore.
The Institute for Digital Medicine (WisDM), Yong Loo Lin School of Medicine, National University of Singapore, Singapore.
SLAS Technol. 2021 Jun;26(3):249-254. doi: 10.1177/24726303211000685. Epub 2021 Mar 27.
This protocol focuses on the culture of cells harvested from hepatocellular carcinoma (HCC) patient-derived xenografts (PDXs) as organoids using a cellulosic macroporous sponge scaffold. Compared with many other epithelial cancer types, the viability of HCC cells directly derived from patients or PDX models is notoriously challenging to maintain in vitro. We previously developed a macroporous sponge scaffold uniquely designed to provide biochemical and mechanical cues that support the culture of normal hepatocytes as spheroids with maintained functionality. Leveraging our success using this sponge scaffold to maintain normal hepatocytes in vitro, we recently demonstrated that a similar sponge scaffold enables the maintenance of HCC PDX cells as organoids with preserved viability, molecular features, and heterogeneity.
本方案重点介绍了使用纤维素大孔海绵支架培养源自肝癌（HCC）患者来源异种移植（PDX）的细胞类器官的方法。与许多其他上皮癌类型相比，直接从患者或 PDX 模型中获得的 HCC 细胞在体外的存活率非常难以维持。我们之前开发了一种大孔海绵支架，其独特设计提供了生化和机械线索，支持正常肝细胞以保持功能的球体形式进行培养。利用我们使用这种海绵支架在体外维持正常肝细胞的成功经验，我们最近证明，类似的海绵支架可以使 HCC PDX 细胞作为类器官保持活力、分子特征和异质性。
