Suzuki Kazuyuki, Kondo Nao, Takagi Kaede, Nishikawa Akitoyo, Murakami Yoshiki, Otsuka Marina, Tsukano Kenji, Ikeda Keiko, Funakura Hisashi, Yasutomi Ichiro, Kawamoto Satoshi
The School of Veterinary Medicine, Rakuno Gakuen University, 582 Midorimachi, Bunnkyoudai, Ebetsu, Hokkaido 069-8501, Japan.
Arekinai Livestock Veterinary Clinic, Hokkaido Higashi Agricultural Mutual Aid Association, 177-3 Kita1senn, Aza Arekinaigenya, Shibecha-cho, Kawakami-gun, Hokkaido 088-2272, Japan.
J Vet Med Sci. 2021 May 9;83(5):767-774. doi: 10.1292/jvms.21-0001. Epub 2021 Mar 26.
Point-of-care (POC) devices that veterinary practitioners can use to easily and rapidly measure blood ionized calcium (iCa) levels in cows immediately after withdrawing a blood sample on the dairy farm are needed. Aims of present studies was to compare the commercially available ion-selective electrode handheld iCa meter (bovine blood iCa checker) with the benchtop blood gas analyzer GEM premier 3500 and handheld analyzer i-STAT 1. Sixty-two paired-point whole blood samples were obtained from three cows with hypocalcemia experimentally induced by Na-EDTA infusion. Whole blood samples were also obtained from the 36 cows kept on a farm in field conditions. The results using the bovine blood iCa checker correlated with those using the GEM premier 3500 and i-STAT 1. Bovine blood iCa checker was "compatible" with the GEM premier 3500 and i-STAT 1 because the frequency of differences between the measurements within ± 20% of the mean were 100% (65/65, >75%) and 90.8% (59/65, >75%), respectively. In the field trial, the blood iCa concentration measured by the bovine blood Ca checker was significantly positively correlated with that measured by the i-STAT 1 portable analyzer. Bovine blood iCa checker was "compatible" with the i-STAT 1 because the frequency of differences between the measurements within ± 20% of the mean was 100% (36/36, >75%). Results from these findings, the bovine blood iCa checker may be applied as a simplified system to measure the iCa concentration in bovine whole blood.
需要一种兽医从业者在奶牛场采集血样后能够轻松快速地测量奶牛血液中离子钙（iCa）水平的即时检测（POC）设备。本研究的目的是将市售的离子选择电极手持式iCa测量仪（牛血iCa检测仪）与台式血气分析仪GEM premier 3500和手持式分析仪i-STAT 1进行比较。从3头通过输注Na-EDTA实验诱导低钙血症的奶牛身上获取了62对全血样本。还从一个农场的36头奶牛身上获取了全血样本。使用牛血iCa检测仪得到的结果与使用GEM premier 3500和i-STAT 1得到的结果相关。牛血iCa检测仪与GEM premier 3500和i-STAT 1 “兼容”，因为测量值之间差异在平均值±20%以内的频率分别为100%（65/65，>75%）和90.8%（59/65，>75%）。在现场试验中，牛血Ca检测仪测量的血iCa浓度与i-STAT 1便携式分析仪测量的结果显著正相关。牛血iCa检测仪与i-STAT 1 “兼容”，因为测量值之间差异在平均值±20%以内的频率为100%（36/36，>75%）。基于这些研究结果，牛血iCa检测仪可作为一种简化系统用于测量牛全血中的iCa浓度。
