Telesnina G N, Sazykin Iu O, Zviagil'skaia R A, Dmitrieva S V, Krakhmaleva I N
Antibiot Khimioter. 1988 Mar;33(3):190-6.
A procedure for preparing stable A. chrysogenum protoplasts capable of 60 per cent regeneration was developed. Two morphogenetic types of the regeneration were detected. The variants isolated after the protoplast regeneration were characterized by wide ranges of morphological variation. Capacity for the antibiotic production varied from 60 to 160 per cent of the activity of the starting strain. A procedure for isolating functionally active mitochondria from protoplasts of A. chrysogenum was also developed. Their main bioenergetic parameters were studied. In the respiratory chain of the A. chrysogenum mitochondria there were detected three conjugation sites of oxidative phosphorylation.
开发出了一种制备稳定的产黄青霉原生质体的方法，其再生率可达60%。检测到两种形态发生类型的再生。原生质体再生后分离出的变体具有广泛的形态变异范围。抗生素生产能力在起始菌株活性的60%至160%之间变化。还开发出了一种从产黄青霉原生质体中分离功能活性线粒体的方法。对其主要生物能量参数进行了研究。在产黄青霉线粒体的呼吸链中检测到三个氧化磷酸化偶联位点。
