Han Bing, Chen Chi, Chen Yi, Wang Ningjian, Yu Jie, Cheng Jing, Chen Yingchao, Zhu Chunfang, Lu Yingli
Institute and Department of Endocrinology and Metabolism, Shanghai Ninth People's Hospital Affiliated to Shanghai Jiaotong University School of Medicine, Shanghai, China.
Eur Thyroid J. 2021 Mar;10(1):79-85. doi: 10.1159/000506701. Epub 2020 Apr 21.
BACKGROUND/AIMS: Hashimoto thyroiditis, characterized by positive thyroid peroxidase antibodies (TPOAbs), is caused by the interaction of genetic and environment factors. The aim of this study was to clarify the interaction of gene polymorphisms and iodine intake in the incidence of TPOAb positivity.
1,733 subjects were included in this study. Genomic DNA was extracted from peripheral blood white cells. Four SNPs (rs11675434 [TPO], rs3094228 [HCP5], rs9277555 [HLA-DPB1], and rs301799 [RERE]) were selected for genotyping. Weighted TPOAb genetic risk score (GRS) was calculated based on these 4 SNPs. Thyroid hormones and autoimmune antibodies (TPOAb and thyroglobulin antibody) were determined using the electrochemiluminescence immunoassay method.
The mean serum thyrotropin level in TPOAb-positive subjects was higher than in TPOAb-negative subjects ( < 0.01). Genotype GG of rs9277555 was associated with an increased risk of TPOAb positivity (OR = 1.64, 5-95% CI 1.09, 2.47, = 0.02). Genotype TT of rs11675434 showed marginal increased risk of TPOAb positivity (OR = 1.57, 5-95% CI 1.01, 2.43, = 0.048). Logistic regression analysis showed TPOAb-GRS and rs9277555 were associated with TPOAb positivity (OR = 5.09, 5-95% CI 1.30, 19.91, = 0.02 and OR = 1.30, 5-95% CI 1.05, 1.61, = 0.02). Subjects with a high TPOAb-GRS had a 52% increased risk of TPOAb positivity compared to subjects with a low TPOAb-GRS (OR 1.52, 5-95% CI 1.05, 2.21, = 0.03).
TPOAb-GRS was associated with an increased risk of TPOAb positivity in a Chinese Han population. This effect might be attribute to rs9277555.
背景/目的：桥本甲状腺炎以甲状腺过氧化物酶抗体（TPOAb）阳性为特征，由遗传和环境因素相互作用引起。本研究旨在阐明基因多态性与碘摄入量在TPOAb阳性发生率中的相互作用。
本研究纳入1733名受试者。从外周血白细胞中提取基因组DNA。选择4个单核苷酸多态性（SNP）（rs11675434 [TPO]、rs3094228 [HCP5]、rs9277555 [HLA-DPB1]和rs301799 [RERE]）进行基因分型。基于这4个SNP计算加权TPOAb遗传风险评分（GRS）。采用电化学发光免疫分析法测定甲状腺激素和自身抗体（TPOAb和甲状腺球蛋白抗体）。
TPOAb阳性受试者的血清促甲状腺激素平均水平高于TPOAb阴性受试者（<0.01）。rs9277555的基因型GG与TPOAb阳性风险增加相关（OR = 1.64，5 - 95%CI 1.09，2.47，= 0.02）。rs11675434的基因型TT显示TPOAb阳性风险略有增加（OR = 1.57，5 - 95%CI 1.01，2.43，= 0.048）。逻辑回归分析显示TPOAb - GRS和rs9277555与TPOAb阳性相关（OR = 5.09，5 - 95%CI 1.30，19.91，= 0.02；OR = 1.30，5 - 95%CI 1.05，1.61，= 0.02）。与低TPOAb - GRS受试者相比，高TPOAb - GRS受试者的TPOAb阳性风险增加52%（OR 1.52，5 - 95%CI 1.05，2.21，= 0.03）。
在中国汉族人群中，TPOAb - GRS与TPOAb阳性风险增加相关。这种效应可能归因于rs9277555。
