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用于多酚检测的基于响应性微凝胶构建的光学器件。

Optical Devices Constructed From Responsive Microgels for Polyphenols Detection.

作者信息

Wang Jingying, Zhang Xieli, Shi Kaiyao, Zhang Qiang

机构信息

Department of Laboratory, 15189 Accredited Laboratory, Jilin Province Drug Resistance Monitoring Center, China-Japan Union Hospital of Jilin University, Changchun, China.

State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, China.

出版信息

Front Chem. 2021 Mar 11;9:580025. doi: 10.3389/fchem.2021.580025. eCollection 2021.

Abstract

Polyphenols are used as antioxidants in various foods and beverages, which are considered to be a health benefit. The measurement of polyphenols contents is of great interest in food chemistry and health science. This work reported a microgels based photonic device (etalon) to detect polyphenols. Dopamine was used as a model compound of polyphenols. Herein, we proposed a "block" concept for dopamine detection. The dopamine was oxidized and formed dopamine films catalyzed by tyrosinase on the surface of etalon. As the etalon was immersed in ZnCl, the dopamine films blocked the ZnCl diffusion into etalon that caused optical property changes. The film thickness is associated with the concentration of dopamine which can be readout via optical signals.

摘要

多酚类物质在各种食品和饮料中用作抗氧化剂,被认为对健康有益。多酚类物质含量的测定在食品化学和健康科学领域中备受研究中备受关注。这项工作报道了一种基于微凝胶的光子器件(标准具)用于检测多酚类物质。多巴胺被用作多酚类物质的模型化合物。在此,我们提出了一种用于多巴胺检测的“阻断”概念。多巴胺被氧化,并在标准具表面由酪氨酸酶催化形成多巴胺薄膜。当标准具浸入氯化锌中时,多巴胺薄膜会阻断氯化锌扩散到标准具中,从而导致光学性质发生变化。薄膜厚度与多巴胺浓度相关,可通过光学信号读出。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6f7/7991913/ff66e016f8fe/fchem-09-580025-g001.jpg

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