Department of Chemistry, University of Alberta , Edmonton, AB T6G 2G2, Canada.
Physical Sciences Department, MacEwan University , Edmonton, AB T5J 4S2, Canada.
ACS Appl Mater Interfaces. 2016 Oct 12;8(40):27264-27269. doi: 10.1021/acsami.6b11462. Epub 2016 Sep 28.
Ferrocene-modified poly(N-isopropylacrylamide)-based microgels were synthesized, characterized, and used to construct optical devices (etalons). The response of the microgels and etalons to HO was investigated, and we show that both the microgel diameter and the optical properties of the etalons depend on the solution concentration of HO from 0.6 to 35 mM. This behavior is a direct result of the oxidation of ferrocene, which influences the microgel diameter. This was also demonstrated by electrochemical-mediated oxidation/reduction of ferrocene using cyclic voltammetry. We go on to show that these materials could be used to monitor HO that is generated from enzymatic reactions. Specifically, we show that the HO generated from the oxidation of glucose catalyzed by glucose oxidase could be quantified. Finally, the devices can be reused multiple times via a regeneration process. This investigation illustrates the versatility of the etalon system to detect species of broad relevance and how they could potentially be used to quantify products of biological reactions.
二茂铁改性的聚（N-异丙基丙烯酰胺）基微凝胶被合成、表征，并用于构建光学器件（光楔）。研究了微凝胶和光楔对 HO 的响应，我们表明，微凝胶直径和光楔的光学性质都取决于 HO 的溶液浓度，范围从 0.6 到 35 mM。这种行为是二茂铁氧化的直接结果，它影响微凝胶的直径。这也通过使用循环伏安法进行电化学介导的二茂铁氧化/还原得到了证明。我们接着表明，这些材料可以用于监测由酶反应产生的 HO。具体来说，我们表明，葡萄糖氧化酶催化葡萄糖氧化产生的 HO 可以被定量。最后，通过再生过程，该设备可以多次重复使用。这项研究说明了光楔系统检测具有广泛相关性的物种的多功能性，以及它们如何可能用于定量生物反应的产物。
