流动线性二色性支持染色质盐诱导凝聚的手风琴模型。

Flow linear dichroism supports an accordion model for the salt-induced condensation of chromatin.

作者信息

Kubista M, Nielsen P E, Nordén B

机构信息

Department of Physical Chemistry, Chalmers University of Technology, Gothenburg, Sweden.

出版信息

Biochem Pharmacol. 1988 May 1;37(9):1813-4. doi: 10.1016/0006-2952(88)90459-5.

DOI:10.1016/0006-2952(88)90459-5

PMID:3377840

Abstract

The salt-induced condensation of chromatin has been studied with flow-linear dichroism technique using an intercalative dye (methylene blue) to selectively monitor the linker orientation. At low ionic strength both linkers and chromatosomes (with their flat faces) are oriented preferentially parallel to the chromatin fibre axis. With increasing ionic strength both linkers and chromatosomes tilt successively towards a perpendicular orientation. Based on these results and structural considerations, an 'Accordion model' with a pentagonal nucleosomal arrangement is proposed for the salt-induced condensation of chromatin.

摘要

利用插入染料（亚甲蓝）通过流动线性二色性技术研究了盐诱导的染色质凝聚，以选择性监测连接体的取向。在低离子强度下，连接体和核小体（其平面）都优先平行于染色质纤维轴取向。随着离子强度的增加，连接体和核小体都相继向垂直取向倾斜。基于这些结果和结构考虑，提出了一种具有五边形核小体排列的“手风琴模型”来解释盐诱导的染色质凝聚。