基于金纳米颗粒的免疫层析分析法用于水和食品样品检测。

Gold nanoparticle-based immunochromatographic assay for detection in water and food samples.

作者信息

Zeng Lu, Guo Lingling, Wang Zhongxing, Xu Xinxin, Ding Hongliu, Song Shanshan, Xu Liguang, Kuang Hua, Xu Chuanlai

机构信息

State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, People's Republic of China.

International Joint Research Laboratory for Biointerface and Biodetection, and School of Food Science and Technology, Jiangnan University, Wuxi, People's Republic of China.

出版信息

Food Chem X. 2021 Mar 5;9:100117. doi: 10.1016/j.fochx.2021.100117. eCollection 2021 Mar 30.

() is the common infection-causing bacterial pathogen. Conventional methods for the detection of are time-consuming, and therefore, a more rapid analytical method is required. Here, monoclonal antibodies (Mabs) against (CICC 10419) were prepared and based on paired Mabs, an immunochromatographic assay (ICA) was developed. The ICA strip showed a limit of detection of 2.41 × 10 CFU/mL and the linear range of detection was 3.13 × 10-1.0 × 10 CFU/mL. No cross-reactivity was observed when other common Gram-negative and Gram-positive bacteria were used. The analytical performance of the ICA strip indicated that the developed ICA had good specificity and stability. Moreover, the feasibility of the ICA strip was verified by detecting (CICC 10419) in spiked water and food samples. The ICA strip could detect samples contaminated with a low-level of (CICC 10419) after 8 h enrichment.

（）是常见的引起感染的细菌病原体。传统的检测方法耗时较长，因此需要一种更快速的分析方法。在此，制备了针对（中国工业微生物菌种保藏管理中心10419）的单克隆抗体，并基于配对的单克隆抗体开发了一种免疫层析测定法（ICA）。该ICA试纸条的检测限为2.41×10 CFU/mL，检测线性范围为3.13×10 - 1.0×10 CFU/mL。当使用其他常见的革兰氏阴性菌和革兰氏阳性菌时，未观察到交叉反应。ICA试纸条的分析性能表明所开发的ICA具有良好的特异性和稳定性。此外，通过检测加标水和食品样品中的（中国工业微生物菌种保藏管理中心10419）验证了ICA试纸条的可行性。经过8小时富集后，ICA试纸条能够检测到被低水平（中国工业微生物菌种保藏管理中心10419）污染的样品。