一种比浊法基于纸张的传感器，用于检测饲料、鸡肉和鸡蛋样本中的托曲珠利及其代谢物。
A colorimetric paper-based sensor for toltrazuril and its metabolites in feed, chicken, and egg samples.
机构信息
State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, People's Republic of China; International Joint Research Laboratory for Biointerface and Biodetection and School of Food Science and Technology, Jiangnan University, Wuxi, People's Republic of China.
Department of Stomatology, The Second People's Hospital of Wuxi, 68 Zhong Shan Road, Wuxi 214002, People's Republic of China.
出版信息
Food Chem. 2019 Mar 15;276:707-713. doi: 10.1016/j.foodchem.2018.10.047. Epub 2018 Oct 10.
In this study, a sensitive monoclonal antibody (mAb) against toltrazuril (Tol) was developed based on a novel hapten. The 50% inhibitory concentrations (IC) of toltrazuril and its metabolites ranged from 2.19 ng/mL to 4.21 ng/mL. Based on this mAb, a colorimetric paper-based sensor was developed for the rapid screening of Tol and its metabolites in samples. The proposed assay has cutoff values of <20 μg/kg for Tol and 50 μg/kg for Tol sulfone when evaluated with the naked eye, and the results could be obtained in 15 min. Quantitative results were obtained with a strip scan reader, with limits of detection <2.60 μg/kg for Tol and its metabolites in real samples. The sensitivity of both qualitative and quantitative detection meets the European Union requirements. Therefore, this strip assay provides a useful tool for the on-site detection and rapid initial screening of Tol and its derivatives in feed, egg, and chicken samples.
本研究基于一种新型半抗原，开发了一种针对甲苯达唑（Tol）的灵敏单克隆抗体（mAb）。甲苯达唑及其代谢物的 50%抑制浓度（IC）范围为 2.19ng/mL 至 4.21ng/mL。基于该 mAb，开发了一种用于快速筛选样品中 Tol 和其代谢物的比色纸质传感器。该检测方法在肉眼评估时，Tol 的截断值为<20μg/kg，Tol 砜的截断值为 50μg/kg，结果可在 15 分钟内获得。用条带扫描读数器获得定量结果，在实际样品中 Tol 及其代谢物的检测限<2.60μg/kg。定性和定量检测的灵敏度均满足欧盟要求。因此，该条带测定法为现场检测和快速初步筛选饲料、鸡蛋和鸡肉样品中的 Tol 及其衍生物提供了一种有用的工具。
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