Centre for Cell & Developmental Biology, State Key Laboratory of Agrobiotechnology, School of Life Sciences, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China.
College of Health Science and Environmental Engineering, Shenzhen Technology University, Shenzhen, 518000, China.
Plant Signal Behav. 2021 May 4;16(5):1901448. doi: 10.1080/15592324.2021.1901448. Epub 2021 Mar 29.
Plant flowering is crucial for the onset and progression of reproduction processes. The control of flowering time is a sophisticated system with multiple known regulatory mechanisms in plants. Here, we show that MYB117 participates in the flowering time regulation in as mutants exhibited early flowering phenotypes under long-day condition. Transcriptome analysis of mutants revealed 410 differentially expressed genes between wild type and -1 mutants, where selective genes including the ( were further confirmed by qRT-PCR analysis. Further, dual-luciferase and chromatin immunoprecipitation quantitative PCR (ChIP-qPCR) assays showed that MYB117 directly binds to the promoter of to suppress its expression. Taken together, we have revealed the transcriptome profile of mutants and identified MYB117 as a negative regulator in controlling flowering time through regulating the expression of in .
植物开花对于繁殖过程的开始和进行至关重要。开花时间的控制是一个复杂的系统，植物中有多个已知的调控机制。在这里，我们发现 MYB117 参与了 的开花时间调控，因为 突变体在长日照条件下表现出早花表型。对 突变体的转录组分析显示，野生型和 -1 突变体之间有 410 个差异表达基因，其中包括选择性基因，如 （进一步通过 qRT-PCR 分析得到证实。此外，双荧光素酶和染色质免疫沉淀定量 PCR（ChIP-qPCR）实验表明，MYB117 直接结合到 的启动子上，抑制其表达。综上所述，我们揭示了 突变体的转录组图谱，并确定 MYB117 通过调节 在控制开花时间方面是一个负调控因子。
