Department of Veterinary Sciences, University of Turin, largo Braccini 2-5, 10095 Grugliasco, Turin, Italy.
Department of Veterinary Medicine, University of Milan, via dell'Università 6, 26900 Lodi, Italy.
Res Vet Sci. 2021 Mar;135:324-328. doi: 10.1016/j.rvsc.2020.10.008. Epub 2020 Oct 20.
Tumor growth depends on both proliferative and apoptotic rate of neoplastic cells. High proliferation index is a well-known negative prognostic factor in canine lymphomas, whereas little is known about apoptotic activity. We describe proliferative and apoptotic rates in different canine lymphoma subtypes at diagnosis. Flow cytometry (FC) was used to assess the percentage of proliferating cells (Ki67%) and of apoptotic cells (AnnV%) in 128 lymph node (LN) aspirates from dogs with lymphoma. Proliferation/apoptosis ratio (PAR) and turnover index (TI; Ki67% + AnnV%) were then calculated for each case. High-grade B-cell lymphomas showed high values for both Ki67% and AnnV%, low-grade B-cell lymphomas showed low Ki67% and high AnnV%, high-grade T-cell lymphomas showed high Ki67% and low AnnV%, and low-grade T-cell lymphomas showed low levels of both parameters. Lymphoblastic lymphomas had the highest PAR values. High-grade B-cell lymphomas had the highest TI values while small clear cells lymphomas the lowest. The panorama of proliferative and apoptotic activity widely varies among lymphoma subtypes. Our results lay the ground for future clinical and pharmacological studies.
肿瘤的生长依赖于肿瘤细胞的增殖和凋亡速率。高增殖指数是犬淋巴瘤中众所周知的不良预后因素，而凋亡活性的了解则较少。我们描述了不同犬淋巴瘤亚型在诊断时的增殖和凋亡率。流式细胞术（FC）用于评估 128 份来自淋巴瘤犬的淋巴结（LN）抽吸物中增殖细胞（Ki67%）和凋亡细胞（AnnV%）的百分比。然后为每个病例计算增殖/凋亡比（PAR）和周转率指数（TI；Ki67% + AnnV%）。高级别 B 细胞淋巴瘤的 Ki67%和 AnnV%均较高，低级别 B 细胞淋巴瘤的 Ki67%较低而 AnnV%较高，高级别 T 细胞淋巴瘤的 Ki67%较高而 AnnV%较低，低级别 T 细胞淋巴瘤的两个参数水平均较低。淋巴母细胞性淋巴瘤的 PAR 值最高。高级别 B 细胞淋巴瘤的 TI 值最高，而小细胞透明细胞淋巴瘤的 TI 值最低。不同淋巴瘤亚型的增殖和凋亡活性的范围广泛变化。我们的研究结果为未来的临床和药理学研究奠定了基础。
