Sokołowska J, Urbańska K, Giziński S, Wysocka A, Cywińska A, Lechowski R
Pol J Vet Sci. 2015;18(1):113-22. doi: 10.1515/pjvs-2015-0015.
Survivin is a member of apoptosis inhibiting proteins family. Apart from its antiapoptotic activity it plays a critical role in regulating the cell cycle and mitosis. It is overexpressed in most human malignancies. While the prognostic significance of survivin expression is widely investigated in human non-Hodgkin's lymphomas, little is known about its expression in canine lymphomas. The aim of the study was to evaluate the expression of survivin in canine lymphomas in relation to proliferation markers (mitotic index and percentage of Ki67-positive cells). Survivin was found in all examined lymphomas belonging to 6 different morphological subtypes with nuclear immunoreactivity. In most of lymphomas (18/25) survivin expression ranged 10%-25% of positive cells. Only single cases had lower (0-10% positive cells, 1/25) or higher (25-50% and > 50% positive cells, 5/25 and 1/25, respectively) index of survivin. Neither mitotic index nor proliferative index correlated with survivin expression when the values quantified randomly in whole specimens were compared. However, when survivin expression were quantified in selected tumor areas of low and high proliferation activity the high correlations between survivin expression and proliferation index were found. The results indicated that survivin is commonly expressed in canine lymphomas. Nuclear labelling together with the relation of its expression and proliferative activity in highly proliferative areas of neoplastic tissue suggest a potential role of survivin in cell cycle activation in canine lymphoma cells. However, further studies of the relation between expression of survivin and other proteins involved in cell cycle regulation are needed. Moreover, the results suggest that survivin may pose the therapeutic target in canine lymphomas.
生存素是凋亡抑制蛋白家族的一员。除了其抗凋亡活性外，它在调节细胞周期和有丝分裂中起着关键作用。它在大多数人类恶性肿瘤中过度表达。虽然生存素表达的预后意义在人类非霍奇金淋巴瘤中得到了广泛研究，但关于其在犬淋巴瘤中的表达却知之甚少。本研究的目的是评估生存素在犬淋巴瘤中的表达与增殖标志物（有丝分裂指数和Ki67阳性细胞百分比）的关系。在所有检查的属于6种不同形态亚型的淋巴瘤中均发现了具有核免疫反应性的生存素。在大多数淋巴瘤（18/25）中，生存素表达的阳性细胞范围为10%-25%。只有个别病例的生存素指数较低（0-10%阳性细胞，1/25）或较高（25-50%和>50%阳性细胞，分别为5/25和1/25）。当比较整个标本中随机量化的值时，有丝分裂指数和增殖指数均与生存素表达无关。然而，当在低增殖活性和高增殖活性的选定肿瘤区域量化生存素表达时，发现生存素表达与增殖指数之间存在高度相关性。结果表明，生存素在犬淋巴瘤中普遍表达。核标记及其在肿瘤组织高增殖区域的表达与增殖活性之间的关系表明，生存素在犬淋巴瘤细胞的细胞周期激活中可能发挥潜在作用。然而，需要进一步研究生存素表达与其他参与细胞周期调节的蛋白质之间的关系。此外，结果表明生存素可能是犬淋巴瘤的治疗靶点。
