Department of Bioengineering and Therapeutic Sciences, California Institute for Quantitative Biosciences, University of California.
Department of Bioengineering and Therapeutic Sciences, California Institute for Quantitative Biosciences, University of California; Chan Zuckerberg Biohub;
J Vis Exp. 2021 Mar 9(169). doi: 10.3791/62248.
Reactions performed in monodispersed droplets afford enhanced accuracy and sensitivity compared to equivalent ones performed in bulk. However, the requirement of microfluidics to form controlled droplets imposes a barrier to non-experts, limiting their use. Here, we describe particle templated emulsification, an approach to generate monodisperse droplets without microfluidics. Using templating hydrogel spheres, we encapsulate samples in monodispersed droplets by simple vortexing. We demonstrate the approach by using it to perform microfluidic-free digital PCR.
在单分散液滴中进行反应比在 bulk 中进行等效反应具有更高的准确性和灵敏度。然而，微流控技术形成受控液滴的要求对非专业人士构成了障碍，限制了它们的使用。在这里，我们描述了一种无需微流控技术即可生成单分散液滴的方法，即颗粒模板乳化法。我们使用模板化水凝胶球，通过简单的涡旋将样品包封在单分散液滴中。我们通过使用该方法进行无微流控数字 PCR 来演示该方法。
