US Department of Agriculture, Agricultural Research Service Center for Medical, Agricultural, & Veterinary Entomology.
US Department of Agriculture, Agricultural Research Service Center for Medical, Agricultural, & Veterinary Entomology;
J Vis Exp. 2021 Mar 12(169). doi: 10.3791/62260.
The control of such human diseases as dengue, Zika, and chikungunya relies on the control of their vector, the Aedes aegypti mosquito, because there is no prevention. Control of mosquito vectors can rely on chemicals applied to the immature and adult stages, which can contribute to the mortality of non-targets and more importantly, lead to insecticide resistance in the vector. The sterile insect technique (SIT) is a method of controlling populations of pests through the release of sterilized adult males that mate with wild females to produce non-viable offspring. This paper describes the process of producing sterile males for use in an operational SIT program for the control of Aedes aegypti mosquitoes. Outlined here are the steps used in the program including rearing and maintaining a colony, separating male and female pupae, irradiating and marking adult males, and shipping Aedes aegypti males to the release site. Also discussed are procedural caveats, program limitations, and future objectives.
登革热、寨卡和基孔肯雅热等人类疾病的控制依赖于其载体埃及伊蚊的控制，因为目前尚无预防方法。控制蚊虫媒介可以依靠应用于幼虫和成虫阶段的化学物质，这可能导致非目标物的死亡，更重要的是，导致媒介对杀虫剂产生抗性。 不育昆虫技术 (SIT) 是通过释放绝育的雄性成虫来控制害虫种群的方法，这些雄性成虫与野生雌性成虫交配产生无活力的后代。本文描述了用于控制埃及伊蚊的运营 SIT 计划中生产不育雄性的过程。这里概述了该计划中使用的步骤，包括饲养和维持一个群体、分离雄性和雌性蛹、辐照和标记成年雄性以及将埃及伊蚊雄性运送到释放地点。还讨论了程序注意事项、计划限制和未来目标。
