Department of Histology and Embryology, Akdeniz University School of Medicine, Antalya, Turkey.
Department of Neurology, Antalya Research and Training Hospital, Neurology Clinic, Antalya, Turkey.
Brain Inj. 2021 Jun 7;35(7):850-862. doi: 10.1080/02699052.2021.1906948. Epub 2021 Mar 29.
: A decrease in the blood flow below a current level in the brain results in ischemia. Studies demonstrated that human trophoblast progenitor cells (hTPCs) contribute to the treatment of many diseases. Therefore, hTPCs might be a promising source to repair ischemia in cerebral ischemia models. For this purpose, we evaluated the expression of many neurogenesis markers by performing hTPC transplantation after focal cerebral ischemia in rats.
: hTPCs, isolated from the term placentae, were characterized by immunofluorescent staining and differentiated into neuron-like cells. Differentiation was confirmed with immunostaining of GFAP and NeuN proteins. Cerebral ischemia models were generated in rats via middle cerebral artery occlusion and, after 24 hours, hTPCs were injected via the tail vein. Animals were sacrificed on day 3 or day 11. Immunohistochemical analysis was performed with proteins associated with neurogenesis and neuronal development, such as DLX2, DLX5, LHX6, NGN1, and NGN2, Olig1, Olig2, and PDGFRα.
: According to our results, hTPCs may alleviate ischemic damage in the brain and contribute to the neurogenesis after ischemia.
: Based on our findings, this topic should be further investigated as the hTPC-based therapies may be a reliable source that can be used in the treatment of stroke and ischemia.
大脑血流低于当前水平会导致缺血。研究表明，人滋养层祖细胞（hTPC）有助于治疗许多疾病。因此，hTPC 可能是修复脑缺血模型中缺血的有前途的来源。为此，我们通过在大鼠局灶性脑缺血后进行 hTPC 移植，评估了许多神经发生标志物的表达。
从足月胎盘分离的 hTPC 通过免疫荧光染色进行鉴定，并分化为类神经元细胞。通过 GFAP 和 NeuN 蛋白的免疫染色来确认分化。通过大脑中动脉闭塞在大鼠中生成脑缺血模型，并在 24 小时后通过尾静脉注射 hTPC。动物在第 3 天或第 11 天被处死。用与神经发生和神经元发育相关的蛋白质，如 DLX2、DLX5、LHX6、NGN1 和 NGN2、Olig1、Olig2 和 PDGFRα 进行免疫组织化学分析。
根据我们的结果，hTPC 可能减轻大脑中的缺血损伤，并有助于缺血后的神经发生。
基于我们的发现，这个课题应该进一步研究，因为基于 hTPC 的治疗可能是一种可靠的来源，可以用于治疗中风和缺血。
