CONACYT, Centro de Investigación Científica de Yucatán, Mérida, Yucatán, México.
CONACYT, Facultad de Enfermería y Nutrición, Universidad Autónoma de San Luis Potosí, San Luis Potosí, México.
Biomed Mater Eng. 2021;32(4):217-228. doi: 10.3233/BME-218000.
Bovine bone matrix is a natural material that has been used in the treatment of bone lesions. In this study, bovine bone matrix Nukbone® (NKB) was investigated due its osteoconductive and osteoinductive properties. This biomaterial induces CBFA-1 activation and osteogenic differentiation, although the cytokines involved in these processes is still unknown.
The aim of this work was to determine the influence of NKB on the pro-osteoblastic and anti-osteoblastic cytokines secretion from human mesenchymal stem cells (hMSCs).
The hMSCs were cultured onto NKB and cytokines IL-2, IL-4, IL-6, IL-10, IL-12, IFN-γ and TNF-α were analized at 0-14 days by immunoassay. In addition, hemocompatibility of NKB and characterization of hMSCs were evaluated.
NKB induces an increase on pro-osteoblastic cytokine secretion IL-4 and a decrease on anti-osteoblastic cytokine IL-6 secretion, at days 7 and 14 of cell culture. Interestingly, there was no statistical difference between secretion profiles of others cytokines analized.
The up-regulation of IL-4 and down-regulation of IL-6, and the secretion profiles of other cytokines examined in this work, are findings that will contribute to the understanding of the role of NKB, and similar biomaterials, in bone homeostasis and in the osteoblastic differentiation of hMSCs.
牛骨基质是一种天然材料，已被用于治疗骨病变。在这项研究中，由于其具有骨诱导和骨传导特性，研究了牛骨基质 Nukbone®（NKB）。这种生物材料诱导 CBFA-1 激活和成骨细胞分化，尽管涉及这些过程的细胞因子仍不清楚。
本工作旨在确定 NKB 对人骨髓间充质干细胞（hMSCs）促成骨细胞和抗成骨细胞细胞因子分泌的影响。
将 hMSCs 培养在 NKB 上，并在 0-14 天通过免疫分析检测 IL-2、IL-4、IL-6、IL-10、IL-12、IFN-γ 和 TNF-α 细胞因子的分泌。此外，还评估了 NKB 的血液相容性和 hMSCs 的特征。
NKB 在细胞培养的第 7 和 14 天诱导促成骨细胞细胞因子 IL-4 的分泌增加和抗成骨细胞细胞因子 IL-6 的分泌减少。有趣的是，分析的其他细胞因子的分泌谱之间没有统计学差异。
本工作中观察到的 IL-4 上调和 IL-6 下调以及其他细胞因子的分泌谱，将有助于理解 NKB 以及类似生物材料在骨稳态和 hMSCs 成骨细胞分化中的作用。
