Universidad Autónoma de Campeche, Campeche, Mexico.
Universidad Autónoma de Yucatán, Yucatan, Mexico.
Biomed Mater Eng. 2023;34(6):561-575. doi: 10.3233/BME-230027.
Bovine pericardium (BP) is a scaffold widely used in soft tissues regeneration; however, its calcification in contact with glutaraldehyde, represent an opportunity for its application in hard tissues, such as bone in the oral cavity.
To develop and to characterize decellularized and glutaraldehyde-crosslinked bovine pericardium (GC-BP) as a potential scaffold for guided bone regeneration GBR.
BP samples from healthy animals of the bovine zebu breed were decellularized and crosslinked by digestion with detergents and glutaraldehyde respectively. The resulting cell-free scaffold was physical, chemical, mechanical, and biologically characterized thought hematoxylin and eosin staining, DNA quantification, scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), uniaxial tensile test, cell viability and live and dead assay in cultures of dental pulp stem cells (DPSCs).
The decellularization and crosslinking of BP appeared to induce conformational changes of the CLG molecules, which led to lower mechanical properties at the GC-BP scaffold, at the same time that promoted cell adhesion and viability of DPSCs.
This study suggests that the decellularized and GC-BP is a scaffold with the potential to be used promoting DPSCs recruitment, which has a great impact on the dental area.
牛心包（BP）是一种广泛应用于软组织再生的支架；然而，其与戊二醛接触时的钙化，为其在硬组织中的应用提供了机会，如口腔中的骨。
开发和表征脱细胞和戊二醛交联牛心包（GC-BP）作为引导骨再生 GBR 的潜在支架。
从健康的瘤牛种动物的 BP 样本中通过用去污剂和戊二醛分别消化来进行脱细胞和交联。无细胞支架通过苏木精和曙红染色、DNA 定量、扫描电子显微镜（SEM）、傅立叶变换红外光谱（FTIR）、热重分析（TGA）、差示扫描量热法（DSC）、单轴拉伸试验、牙髓干细胞（DPSCs）培养中的细胞活力和死活测定进行物理、化学、机械和生物学表征。
BP 的脱细胞和交联似乎诱导了 CLG 分子的构象变化，这导致 GC-BP 支架的机械性能降低，同时促进了 DPSCs 的黏附和活力。
本研究表明，脱细胞和 GC-BP 是一种具有促进 DPSCs 募集潜力的支架，这对牙科领域有重大影响。
