Department of Animal Science, College of Agriculture, University of Tabriz, Tabriz, Iran.
Department of Animal Science, College of Agriculture, University of Tabriz, Tabriz, Iran.
Anim Reprod Sci. 2021 May;228:106738. doi: 10.1016/j.anireprosci.2021.106738. Epub 2021 Mar 18.
Poloxamer and hydroxyethyl starch have cytoprotective effects. In the present study, effectiveness was evaluated of these compounds as a cryoprotectant for rooster semen. In Experiment 1 (E1), poloxamer 188 (1%, P188), poloxamer 407 (1%, P407), and control groups were compared after sperm cryopreservation. Experiment 2 (E2) was conducted with 3%, 5%, and 7% of hydroxyethyl starch (H3, H5, H7), also combined with P188 (H3P188, H5P188, H7P188), based on results from E1. Sperm motility was assessed using CASA, abnormal forms and hypo-osmotic swelling (HOS) were evaluated using microscopy, and viability, apoptotic-like changes, and mitochondrial activity were determined using flow cytometry. In E2, there were assessments of fertility and hatching capacity. Results from E1 indicated total and progressive motility, velocity, membrane functionality, viability, and mitochondrial activity were greater with inclusion of P188 in semen extender, with less apoptotic-like changes (P < 0.05). In E2, HES inclusion in semen extender improved total motility, membrane functionality, and mitochondrial activity (P < 0.05), especially H5, which also markedly increased sperm viability and decreased apoptotic-like changes. The combination of P188 with HES increased sperm quality overall, with inclusion of H5P188 resulting in increases of progressive motility and VSL (P < 0.05). The H5 inclusion also increased proportion of fertilized eggs (P < 0.05). Furthermore, the combination of HES and P188 increased proportions of fertilized and hatched eggs compared with the control, with inclusion of H5P188 having the greatest effects. In conclusion, supplementation of semen extender with H5P188 increases post-thawing quality and fertility of rooster sperm, being a safe and effective method for the poultry industry.
泊洛沙姆和羟乙基淀粉具有细胞保护作用。在本研究中，评估了这些化合物作为鸡精液冷冻保护剂的效果。在实验 1（E1）中，比较了冷冻保存后的聚氧乙烯 188（1%，P188）、聚氧乙烯 407（1%，P407）和对照组。实验 2（E2）是在 E1 的基础上，用 3%、5%和 7%的羟乙基淀粉（H3、H5、H7）进行的，也与 P188（H3P188、H5P188、H7P188）结合进行。使用 CASA 评估精子运动，使用显微镜评估异常形态和低渗肿胀（HOS），使用流式细胞术评估活力、凋亡样变化和线粒体活性。在 E2 中，评估了受精和孵化能力。E1 的结果表明，在精液稀释液中加入 P188 可提高总活力、前向运动活力、速度、膜功能、活力和线粒体活性，凋亡样变化减少（P<0.05）。在 E2 中，羟乙基淀粉在精液稀释液中的添加提高了总活力、膜功能和线粒体活性（P<0.05），特别是 H5，还显著增加了精子活力，减少了凋亡样变化。P188 与羟乙基淀粉的组合总体上提高了精子质量，加入 H5P188 可提高前向运动活力和 VSL（P<0.05）。H5 的添加还增加了受精卵的比例（P<0.05）。此外，与对照组相比，羟乙基淀粉和 P188 的组合增加了受精卵和孵化卵的比例，而 H5P188 的效果最大。综上所述，在精液稀释液中添加 H5P188 可提高鸡精子解冻后的活力和受精率，是家禽业安全有效的方法。
