Stuermer E K, Besser M, Brill F, Geffken M, Plattfaut I, Severing A L, Wiencke V, Rembe J D, Naumova E A, Kampe A, Debus S, Smeets R
Dept. of Vascular Medicine, University Heart Center, University Medical Center Hamburg-Eppendorf (UKE), Martini Street 52, 20246, Hamburg, Germany.
Dpt. of Translational Wound Research, Centre for Biomedical Education and Research (ZBAF), Witten/Herdecke University, Stockumer Street 10, Witten, Germany.
Int J Hyg Environ Health. 2021 May;234:113744. doi: 10.1016/j.ijheh.2021.113744. Epub 2021 Mar 26.
Biofilms are one of the greatest challenges in today's treatment of chronic wounds. While antimicrobials kill platonic bacteria within seconds, they are rarely able to harm biofilms. In order to identify effective substances for antibacterial therapy, cost-efficient, standardized and reproducible models that aim to mimic the clinical situation are required. In this study, two 3D biofilm models based on human plasma with immune cells (lhBIOM) or based on sheep blood (sbBIOM) containing S. aureus or P. aeruginosa, are compared with the human biofilm model hpBIOM regarding their microscopic structure (scanning electron microscopy; SEM) and their bacterial resistance to octenidine hydrochloride (OCT) and a sodium hypochlorite (NaOCl) wound-irrigation solution. The three analyzed biofilm models show little to no reaction to treatment with the hypochlorous solution while planktonic S. aureus and P. aeruginosa cells are reduced within minutes. After 48 h, octenidine hydrochloride manages to erode the biofilm matrix and significantly reduce the bacterial load. The determined effects are qualitatively reflected by SEM. Our results show that both ethically acceptable human and sheep blood based biofilm models can be used as a standard for in vitro testing of new antimicrobial substances. Due to their composition, both fulfill the criteria of a reality-reflecting model and therefore should be used in the approval for new antimicrobial agents.
生物膜是当今慢性伤口治疗中最大的挑战之一。虽然抗菌剂能在几秒钟内杀死游离细菌，但它们很少能对生物膜造成损害。为了确定抗菌治疗的有效物质，需要经济高效、标准化且可重复的模型来模拟临床情况。在本研究中，将两种基于含有免疫细胞的人血浆（lhBIOM）或基于含有金黄色葡萄球菌或铜绿假单胞菌的羊血（sbBIOM）的3D生物膜模型，在微观结构（扫描电子显微镜；SEM）以及它们对盐酸奥替尼啶（OCT）和次氯酸钠（NaOCl）伤口冲洗液的细菌抗性方面，与人类生物膜模型hpBIOM进行比较。三种分析的生物膜模型对次氯酸盐溶液处理几乎没有反应，而游离的金黄色葡萄球菌和铜绿假单胞菌细胞在几分钟内就会减少。48小时后，盐酸奥替尼啶能够侵蚀生物膜基质并显著降低细菌载量。SEM定性地反映了所确定的效果。我们的结果表明，基于人血和羊血的符合伦理规范的生物膜模型都可作为新抗菌物质体外测试的标准。由于它们的组成，两者都符合反映实际情况的模型标准，因此应用于新抗菌剂的审批。
